to the Availability of RIKEN ENU-based gene-driven mutagenesis system (RGDMS) with the target-gene list and available mutant lines.

ENU-based gene-driven mutagenesis: NEXT-GENERATION GENE-TARGETING. [To Japanese Page]

Hot Topics: The RIKEN gene-driven mutagenesis system (RGDMS) is open to public within our capacity at the RIKEN BioResource Center. Established mutant lines are also available to research community. Click here or inquiry to;


[Please note that ENU mutant mice are non LMOs (non living modified organisms).] @




Functional Genomics Research Group at RIKEN Genomic Sciences Center archived ~10,000 G1 mouse sperm during the process of a large-scale ENU mouse mutagenesis during 2000 - 2008. The Population and Quantitative Genomics Team at RIKEN Genomic Sciences Center also constucted genomic DNA archive from the same set of G1 mice and developed a sequence-based screening system to conduct the ENU-based gene-driven mutagenesis. We call these dual archives "RIKEN mutant mouse libary".

The RIKEN Genomic Sciences Center closed March 2008 and the RIKEN mutant mouse library was transferred to RIKEN BioResource Center. To continue the Population and Quantitative Genomics Team's effort, a new team, Mutagenesis and Genomics Team was newly organized at the RIKEN BioResource Center as of April 2008. Now the Mutagenesis and Genomics Team is in charge of the ENU-based gene-driven mouse mutagenesis.



The Mutagenesis and Genomics Team utilizes the frozen sperm G1 mouse resource to conduct the ENU-based gene-driven mutagenesis. In addition to the sperm cryopreservation by the Mouse Functional Genomics Research Teams, we also keep several organs from each G1 male in order to archive genomic DNA from all the G1 males. By utilizing these genetic resources, feasibilities for gene-driven mutagenesis have been investigated. We now archived ~10,000 G1 male sperm and most of their genomic DNAs. We also established a mutation discovery system by using a temperature gradient capillary electrophoresis (TGCE) as a primary mutation screening system [1].

In addition, the Mutagenesis and Genomics Team is trying to accerelate the pace of the mutation discovery by using, for instance, TILLING/Cel1 digestion method, High Resolution Melt (HRM) methods, etc. Furthermore, the next-generation re-sequencing method is under investigation in order to thouroughly identify ENU-induced mutations in genomewide. At present, we are trying to enrich a part of or whole mouse exome and re-sequence by using one of the next-generation sequencers, for instance, Illumina, SOLiD and Helicos, which are currently available at the RIKEN Omics Research Center.

3. Publiations related to the RIKEN ENU-based gene-driven mutagenesis.


Mutation detection/rate/spectrum.

Targeted mutagenesis and established mutant lines.


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