Procedure Immunophenotyping test v.1.0
Pipeline IMPC_adult_v2-LaterOnset Screen Age (week) 16-56w
Parameter Group T cell (panel A)
Parameter CD8 Central Memory T
Strain Condition 1 Pipeline(s) Tested Day
(Age)
IMPC_adult_v2-LaterOnset
C57BL/6NJcl Late ♀: 3  ♂: 3 2015/11/26 - 2016/08/30
(17.07 - 56.93)
Early ♀: 3  ♂: 3
Raw Data

Bartlett test result
Factor df Bartlett's K2 p
condition1 1 3.470274 0.06248095
sex 1 5.320728 0.02107321

Summary statistics
Factor mean median SD SEM N
condition1 - sex
Early(16w) - Female 30.58 31.74 2.37 1.37 3
Early(16w) - Male 32.6 33.36 2.18 1.26 3
Late(56w) - Female 40.2 37.69 4.42 2.55 3
Late(56w) - Male 30.9 30.3 1.36 0.78 3

ANOVA result
Signif. codes ***: <0.001
**: <0.010
*: <0.050
†: <0.100
Factor df Sum Sq. F value Pr(>F) Signif.
condition1 1 47.005 5.9184 0.041024 *
sex 1 39.785 5.0093 0.055585 †
condition1:sex 1 96.277 12.1221 0.008299 **
Residuals 8 63.538

Tukey multiple comparisons of means
Factor: condition1
Level Difference Lower Upper Adjusted p
Late(56w) - Early(16w) 3.96 0.206 7.71 0.041

Factor: condition1:sex
Level Difference Lower Upper Adjusted p
Late(56w):Female - Early(16w):Female 9.623 2.25 16.992 0.0131
Early(16w):Male - Early(16w):Female 2.023 -5.35 9.392 0.8156
Late(56w):Male - Early(16w):Female 0.317 -7.05 7.685 0.9990
Early(16w):Male - Late(56w):Female -7.600 -14.97 -0.231 0.0434
Late(56w):Male - Late(56w):Female -9.307 -16.68 -1.938 0.0157
Late(56w):Male - Early(16w):Male -1.707 -9.08 5.662 0.8777



FM_02_U_barchart.png

comment
CD8 CM T was significantly increased in 56-week-old mice (Late) relative to 16-week-old mice (Early) (p<0.05) in only female.