Procedure Immunophenotyping test v.1.0
Pipeline IMPC_adult_v2-LaterOnset Screen Age (week) 16-56w
Parameter Group NKT cell (panel A)
Parameter DN Naive NKT
Strain Condition 1 Pipeline(s) Tested Day
(Age)
IMPC_adult_v2-LaterOnset
C57BL/6NJcl Late ♀: 3  ♂: 3 2015/11/26 - 2016/08/30
(17.07 - 56.93)
Early ♀: 3  ♂: 3
Raw Data

Bartlett test result
Factor df Bartlett's K2 p
condition1 1 1.434196 0.2310809
sex 1 1.986251 0.1587334

Summary statistics
Factor mean median SD SEM N
condition1 - sex
Early(16w) - Female 0.19 0.2 0.05 0.03 3
Early(16w) - Male 0.05 0.07 0.04 0.02 3
Late(56w) - Female 0.02 0 0.03 0.02 3
Late(56w) - Male 0.04 0 0.07 0.04 3

ANOVA result
Signif. codes ***: <0.001
**: <0.010
*: <0.050
†: <0.100
Factor df Sum Sq. F value Pr(>F) Signif.
condition1 1 0.024300 10.3772 0.01221 *
sex 1 0.010800 4.6121 0.06402 †
condition1:sex 1 0.020833 8.8968 0.01753 *
Residuals 8 0.018733

Tukey multiple comparisons of means
Factor: condition1
Level Difference Lower Upper Adjusted p
Late(56w) - Early(16w) -0.09 -0.154 -0.0256 0.0122

Factor: condition1:sex
Level Difference Lower Upper Adjusted p
Late(56w):Female - Early(16w):Female -0.17333 -0.2999 -0.0468 0.0100
Early(16w):Male - Early(16w):Female -0.14333 -0.2699 -0.0168 0.0276
Late(56w):Male - Early(16w):Female -0.15000 -0.2765 -0.0235 0.0219
Early(16w):Male - Late(56w):Female 0.03000 -0.0965 0.1565 0.8703
Late(56w):Male - Late(56w):Female 0.02333 -0.1032 0.1499 0.9322
Late(56w):Male - Early(16w):Male -0.00667 -0.1332 0.1199 0.9981



FM_02_U_barchart.png

comment
DN Naive NKT was significantly decreased in 56-week-old mice (Late) relative to 16-week-old mice (Early) (p<0.05) in only female.