Procedure Immunophenotyping test v.1.0
Pipeline IMPC_adult_v2-LaterOnset Screen Age (week) 16-56w
Parameter Group NKT cell (panel A)
Parameter CD4 Naive NKT
Strain Condition 1 Pipeline(s) Tested Day
(Age)
IMPC_adult_v2-LaterOnset
C57BL/6NJcl Late ♀: 3  ♂: 3 2015/11/26 - 2016/08/30
(17.07 - 56.93)
Early ♀: 3  ♂: 3
Raw Data

Bartlett test result
Factor df Bartlett's K2 p
condition1 1 2.088905 0.1483724
sex 1 0.4919729 0.4830486

Summary statistics
Factor mean median SD SEM N
condition1 - sex
Early(16w) - Female 0.53 0.5 0.27 0.15 3
Early(16w) - Male 0.5 0.5 0.33 0.19 3
Late(56w) - Female 1.19 1.37 0.51 0.29 3
Late(56w) - Male 1.52 1.55 0.62 0.36 3

ANOVA result
Signif. codes ***: <0.001
**: <0.010
*: <0.050
†: <0.100
Factor df Sum Sq. F value Pr(>F) Signif.
condition1 1 2.14207 10.4479 0.01202 *
sex 1 0.06601 0.3220 0.58600
condition1:sex 1 0.09901 0.4829 0.50679
Residuals 8 1.64020

Tukey multiple comparisons of means
Factor: condition1
Level Difference Lower Upper Adjusted p
Late(56w) - Early(16w) 0.845 0.242 1.45 0.0120



FM_02_U_barchart.png

comment
CD4 Naive NKT was significantly increased in 56-week-old mice (Late) relative to 16-week-old mice (Early) (p<0.05).