Procedure Immunophenotyping test v.1.0
Pipeline IMPC_adult_v2-LaterOnset Screen Age (week) 16-56w
Parameter Group NKT cell (panel A)
Parameter CD4 NKT
Strain Condition 1 Pipeline(s) Tested Day
(Age)
IMPC_adult_v2-LaterOnset
C57BL/6NJcl Late ♀: 3  ♂: 3 2015/11/26 - 2016/08/30
(17.07 - 56.93)
Early ♀: 3  ♂: 3
Raw Data

Bartlett test result
Factor df Bartlett's K2 p
condition1 1 0.2520623 0.6156267
sex 1 0.07539 0.7836448

Summary statistics
Factor mean median SD SEM N
condition1 - sex
Early(16w) - Female 38.49 34.32 8.55 4.94 3
Early(16w) - Male 36.28 33.84 5.11 2.95 3
Late(56w) - Female 29.71 30.38 6.05 3.49 3
Late(56w) - Male 25.31 24.88 3.6 2.08 3

ANOVA result
Signif. codes ***: <0.001
**: <0.010
*: <0.050
†: <0.100
Factor df Sum Sq. F value Pr(>F) Signif.
condition1 1 292.349 7.8624 0.02306 *
sex 1 32.835 0.8831 0.37487
condition1:sex 1 3.619 0.0973 0.76303
Residuals 8 297.464

Tukey multiple comparisons of means
Factor: condition1
Level Difference Lower Upper Adjusted p
Late(56w) - Early(16w) -9.87 -18 -1.75 0.0231



FM_02_U_barchart.png

comment
CD4 NKT was significantly decreased in 56-week-old mice (Late) relative to 16-week-old mice (Early) (p<0.05).