Phenotyping platform of JMC

Phenotyping platform

  In Japan Mouse Clinic, we have developed the two pipelines which are constructed with comprehensive test items.

Pipeline 1

  Comprehensive test items examining metabolic, blood/hematic, osseous /skeletal, cancer, cardiovascular, sensory, skin/fur, and neural /behavioral characteristics, construct pipeline 1. Early primary “Fundamental screen” and late secondary “In depth screen”, which are carried out depending on the results of “Fundamental screen”, are systematically performed.

Fundamental screen Test ID Screens Methods Age(weeks)
8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26
P1-01 Morphology/
Behavioral/
Sensory
Modified-SHIRPA
                                     
P1-02 Hematology/
Clinical Chemistry
Hematological test                                      
P1-03 Urinalysis                                      
P1-04 Clinical biochemical test                                      
P1-05 Pathology Autopsy, Histology                                      
In depth screen P1-06 Sensory ABR (Auditory brainstem response)                                      
P1-07 Metabolism IPGTT (Intra-peritoneal glucose tolerance test)                                      
P1-08 Adipocytokine and clinical biochemical test (glucose and adipose related)                                      
P1-09 Sensory Funduscopy                                      
P1-10 Morphology X-ray                                      
P1-11 Metabolism Body fat percentage and Bone Mineral Density (DEXA)                                      
P1-12 Cardiovascular ECG (Electrocardiography)                                      
P1-13 Immunology FACS                                      

 

Pipeline 2

Behavioral
screen
Test ID Screens Methods Age(weeks)
6 7 8 9 10 11 12 13 14
P2-01 Behavior/
Neurology/
Psychiatry
Light/dark transition                  
P2-02 Open-field test                  
P2-03 Crawley’s 3 chamber test                  
P2-04 Home-cage activity                  
P2-05 Y maze                  
P2-06 Fear-conditioning test                  
P2-07 ASR/PPI                  
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