Procedure Immunophenotyping test v.1.0
Pipeline IMPC_adult_v2-LaterOnset Screen Age (week) 16-56w
Parameter Group B cell (panel B)
Parameter pre-B
Strain Condition 1 Pipeline(s) Tested Day
(Age)
IMPC_adult_v2-LaterOnset
C57BL/6NJcl Late ♀: 3  ♂: 3 2015/11/26 - 2016/08/30
(17.07 - 56.93)
Early ♀: 3  ♂: 3
Raw Data

Bartlett test result
Factor df Bartlett's K2 p
condition1 1 0.1721275 0.6782279
sex 1 0.001807581 0.9660876

Summary statistics
Factor mean median SD SEM N
condition1 - sex
Early(16w) - Female 7.29 7.81 1.36 0.79 3
Early(16w) - Male 7.31 6.96 0.89 0.51 3
Late(56w) - Female 10.47 10.03 0.77 0.44 3
Late(56w) - Male 10.66 10.47 1.08 0.63 3

ANOVA result
Signif. codes ***: <0.001
**: <0.010
*: <0.050
†: <0.100
Factor df Sum Sq. F value Pr(>F) Signif.
condition1 1 31.948 29.0523 0.0006538 ***
sex 1 0.034 0.0310 0.8645305
condition1:sex 1 0.024 0.0221 0.8855070
Residuals 8 8.797

Tukey multiple comparisons of means
Factor: condition1
Level Difference Lower Upper Adjusted p
Late(56w) - Early(16w) 3.26 1.87 4.66 0.000654



FM_02_U_barchart.png

comment
pre-B was significantly increased in 56-week-old mice (Late) relative to 16-week-old mice (Early) (p<0.001).