Procedure Immunophenotyping test v.1.0
Pipeline IMPC_adult_v2-LaterOnset Screen Age (week) 16-56w
Parameter Group B cell (panel B)
Parameter Follicular B
Strain Condition 1 Pipeline(s) Tested Day
(Age)
IMPC_adult_v2-LaterOnset
C57BL/6NJcl Late ♀: 3  ♂: 3 2015/11/26 - 2016/08/30
(17.07 - 56.93)
Early ♀: 3  ♂: 3
Raw Data

Bartlett test result
Factor df Bartlett's K2 p
condition1 1 0.1816708 0.6699413
sex 1 0.004742947 0.9450938

Summary statistics
Factor mean median SD SEM N
condition1 - sex
Early(16w) - Female 87.38 86.67 1.73 1 3
Early(16w) - Male 88.5 88.77 0.67 0.39 3
Late(56w) - Female 83.56 84.11 1.72 0.99 3
Late(56w) - Male 84.14 83.6 1.83 1.05 3

ANOVA result
Signif. codes ***: <0.001
**: <0.010
*: <0.050
†: <0.100
Factor df Sum Sq. F value Pr(>F) Signif.
condition1 1 50.103 20.5617 0.001913 **
sex 1 2.185 0.8965 0.371436
condition1:sex 1 0.219 0.0898 0.772128
Residuals 8 19.494

Tukey multiple comparisons of means
Factor: condition1
Level Difference Lower Upper Adjusted p
Late(56w) - Early(16w) -4.09 -6.16 -2.01 0.00191



FM_02_U_barchart.png

comment
FOB was significantly decreased in 56-week-old mice (Late) relative to 16-week-old mice (Early) (p<0.01).