Genotype Viewer

Procedure	Clinical Blood Chemistry Clinical biochemical test v2
Pipeline	IMPC_adult_v2-LaterOnset	Screen Age (week)	16-56w
Parameter	Total protein (TP:g/L)

Strain	Condition 1	Pipeline(s)	Tested Day (Age)
Strain	Condition 1	IMPC_adult_v2-LaterOnset	Tested Day (Age)
C57BL/6NJcl	Late	♀: 11 ♂: 9	2015/11/25 - 2016/08/31 (16.5 - 56.93)
C57BL/6NJcl	Early	♀: 14 ♂: 12	2015/11/25 - 2016/08/31 (16.5 - 56.93)

Raw Data

Bartlett test result
Factor	df	Bartlett's K²	p
condition1	1	6.963955	0.008316801
sex	1	7.474998	0.006256165

Summary statistics
Factor	mean	median	SD	SEM	N
condition1 - sex	mean	median	SD	SEM	N
Early(16w) - Female	44.95	45.4	2.8	0.75	14
Early(16w) - Male	45.03	44.9	2.68	0.77	12
Late(56w) - Female	45.92	45.5	2.73	0.82	11
Late(56w) - Male	51.83	52.2	4.83	1.61	9

ANOVA result
Factor	df	Sum Sq.	F value	Pr(>F)	Signif.
Signif. codes *: <0.001 : <0.010 *: <0.050 †: <0.100
condition1	1	148.26	14.0902	0.0005292	***
sex	1	77.92	7.4051	0.0094245	**
condition1:sex	1	95.32	9.0597	0.0044066	**
Residuals	42	441.92

Tukey multiple comparisons of means
Factor: condition1
Level	Difference	Lower	Upper	Adjusted p
Late(56w) - Early(16w)	3.59	1.64	5.54	0.00058

Factor: sex
Level	Difference	Lower	Upper	Adjusted p
Male - Female	2.61	0.675	4.55	0.00943

Factor: condition1:sex
Level	Difference	Lower	Upper	Adjusted p
Late(56w):Female - Early(16w):Female	0.9682	-2.53	4.46	8.80e-01
Early(16w):Male - Early(16w):Female	0.0833	-3.33	3.50	1.00e+00
Late(56w):Male - Early(16w):Female	6.8833	3.18	10.59	6.82e-05
Early(16w):Male - Late(56w):Female	-0.8848	-4.51	2.74	9.14e-01
Late(56w):Male - Late(56w):Female	5.9152	2.02	9.82	1.17e-03
Late(56w):Male - Early(16w):Male	6.8000	2.97	10.63	1.35e-04

comment
TP was significantly increased in 56-week-old mice (Late) relative to 16-week-old mice (Early) (p<0.001) in only male.