Genotype Viewer

Procedure	Clinical Blood Chemistry Clinical biochemical test v2
Pipeline	IMPC_adult_v2-LaterOnset	Screen Age (week)	16-56w
Parameter	Alpha-amylase (AMY)

Strain	Condition 1	Pipeline(s)	Tested Day (Age)
Strain	Condition 1	IMPC_adult_v2-LaterOnset	Tested Day (Age)
C57BL/6NJcl	Late	♀: 11 ♂: 9	2015/11/25 - 2016/08/31 (16.5 - 56.93)
C57BL/6NJcl	Early	♀: 14 ♂: 12	2015/11/25 - 2016/08/31 (16.5 - 56.93)

Raw Data

Bartlett test result
Factor	df	Bartlett's K²	p
condition1	1	11.47233	0.0007064022
sex	1	18.77495	1.470866e-05

Summary statistics
Factor	mean	median	SD	SEM	N
condition1 - sex	mean	median	SD	SEM	N
Early(16w) - Female	672.64	667.5	50.99	13.63	14
Early(16w) - Male	847	845	107.13	30.92	12
Late(56w) - Female	811.27	838	68.83	20.75	11
Late(56w) - Male	1177.33	1148	248.08	82.69	9

ANOVA result
Factor	df	Sum Sq.	F value	Pr(>F)	Signif.
Signif. codes *: <0.001 : <0.010 *: <0.050 †: <0.100
condition1	1	576569	34.6044	5.860e-07	***
sex	1	756731	45.4174	3.435e-08	***
condition1:sex	1	103005	6.1821	0.01697	*
Residuals	42	699791

Tukey multiple comparisons of means
Factor: condition1
Level	Difference	Lower	Upper	Adjusted p
Late(56w) - Early(16w)	223	145	300	7.56e-07

Factor: sex
Level	Difference	Lower	Upper	Adjusted p
Male - Female	257	180	335	3.44e-08

Factor: condition1:sex
Level	Difference	Lower	Upper	Adjusted p
Late(56w):Female - Early(16w):Female	138.6	-0.489	278	5.11e-02
Early(16w):Male - Early(16w):Female	174.4	38.523	310	7.12e-03
Late(56w):Male - Early(16w):Female	504.7	357.169	652	8.93e-11
Early(16w):Male - Late(56w):Female	35.7	-108.402	180	9.10e-01
Late(56w):Male - Late(56w):Female	366.1	210.867	521	8.38e-07
Late(56w):Male - Early(16w):Male	330.3	178.077	483	4.45e-06

comment
AMY was significantly increased in 56-week-old mice (Late) relative to 16-week-old mice (Early) (p<0.001) in only male.