Procedure Hematology Blood collection for hematological test v2
Pipeline IMPC_adult_v2-LaterOnset Screen Age (week) 16-56w
Parameter Absolute number of neutrophils (NEUT number)
Strain Condition 1 Pipeline(s) Tested Day
(Age)
IMPC_adult_v2-LaterOnset
C57BL/6NJcl Late ♀: 10  ♂: 8 2015/11/24 - 2016/08/29
(16.36 - 56.64)
Early ♀: 14  ♂: 11
Raw Data

Bartlett test result
Factor df Bartlett's K2 p
condition1 1 1.472004 0.2250298
sex 1 5.162988 0.02307317

Summary statistics
Factor mean median SD SEM N
condition1 - sex
Early(16w) - Female 0.83 0.8 0.18 0.05 14
Early(16w) - Male 0.71 0.7 0.14 0.04 11
Late(56w) - Female 0.55 0.48 0.25 0.08 10
Late(56w) - Male 0.67 0.71 0.17 0.06 8

ANOVA result
Signif. codes ***: <0.001
**: <0.010
*: <0.050
†: <0.100
Factor df Sum Sq. F value Pr(>F) Signif.
condition1 1 0.31958 9.0882 0.004507 **
sex 1 0.00259 0.0737 0.787402
condition1:sex 1 0.15241 4.3341 0.043965 *
Residuals 39 1.37142

Tukey multiple comparisons of means
Factor: condition1
Level Difference Lower Upper Adjusted p
Late(56w) - Early(16w) -0.175 -0.292 -0.0576 0.00449

Factor: condition1:sex
Level Difference Lower Upper Adjusted p
Late(56w):Female - Early(16w):Female -0.2823 -0.491 -0.0739 0.00427
Early(16w):Male - Early(16w):Female -0.1175 -0.320 0.0853 0.41575
Late(56w):Male - Early(16w):Female -0.1568 -0.380 0.0662 0.25038
Early(16w):Male - Late(56w):Female 0.1648 -0.055 0.3847 0.20126
Late(56w):Male - Late(56w):Female 0.1255 -0.113 0.3642 0.50031
Late(56w):Male - Early(16w):Male -0.0393 -0.273 0.1945 0.96900



FM_02_U_barchart.png

comment
NEUT number was significantly decreased in 56-week-old mice (Late) relative to 16-week-old mice (Early) in only female (p<0.01).