Japan Mouse Clinic
Japanese
Clinic inspection(pipeline01)



Pipeline 1
  Comprehensive test items examining metabolic, blood/hematic, osseous /skeletal, cancer, cardiovascular, sensory,skin/fur, and neural / behavioral characteristics, construct pipeline 1. Early primary "Fundamental screen" and late secondary "In depth screen" are systematically performed.




  P1-01: Modified-SHIRPA ( 8w ) sop_b.png   sop_b.png

  The modified SHIRPA is a simple method for evaluating the characteristic behaviors and morphologies of mice.

  52 items about various mouse behaviors and morphologies, and its sensory responses are tested by visual inspection and manual handling using simple equipments.
  


  P1-02: Hematological test ( 9w ) sop_b.png   sop_b.png

Blood Collection by
retroorbital puncture
(Using EDTA for anti-coagulant)
    In hematological test, 24 parameters of blood count
    (white blood cell count, red blood cell count, platelet, etc)
    are measured with about 200 micro-L of mouse blood.
Analysis by automatic
hematology analyzer
(ADVIA2120i, SIEMENS)


  P1-03: Urinalysis ( 10w ) sop_b.png  

        Urine tests are performed using dip sticks (Arkray Factory). The list of the tests is the following table.

Test paper: Aution Sticks 7EA
(ARKRAY, Inc)
  


  P1-04: Clinical biochemical test (11w) sop_b.png   sop_b.png

Blood Collection by retro-orbital puncture
(Using Coagulant Wako for coagulation reagent)

Centrifuge for serum separation
(Use blood clot for HbA1c measurement)


Analysis by automatic clinical biochemical analyzer(JCA-BM6070, JEOL)

  In clinical biochemical test, 21 parameters of serum biochemical tests (LDH, AST, Glu, T-CHO, etc) are measured with mouse serum.


  P1-05: Macroscopic test ( 26w ) sop_b.png  

  After the early-onset phenotype analyses of mice in 13 weeks after birth, pathological phenotype analyses were commenced and to be continued until 26weeks of age. During this period, anatomical and histological test (option) .



  P1-06: ABR(Auditory brainstem response)( 13w ) sop_b.png   sop_b.png

  Measurement of   
  Auditory Brainstem  
  Response (ABR)  
  ABR is sound evoked cumulative electroencephalography of neuronal cell cascades transmitting electric signals to CNS. Measurement of ABR demonstrates quantitative index for auditory functions.


  P1-07: IPGTT (Intra-peritoneal glucose tolerance test)( 14w ) sop_b.png  


GLUCOCARD
(ARKRAY, Inc)
  The glucose tolerance test measures the clearance of a standardized glucose load from the body.
  Mice are fasted for approximately 16 hours (overnight), before a glucose solution is administered intraperitoneally.
  Subsequent blood glucose is measured at different time points during the following 2 hours using a portable blood glucose monitor (GLUCOCARD; ARKRAY Inc.).


  P1-08: Adipocytokine and clinical biochemical test( 18w ) sop_b.png  

Blood Collection by retro-orbital puncture
(Using Coagulant Wako for coagulation reagent)

adipocytokine

Clinical biochemistry

Use blood clot for HbA1c measurement
Centrifuge for
serum separation


Analysis by multiplex suspension array system
(BioPlex, Bio-Rad)



Analysis by automatic clinical biochemical analyzer
(JCA-BM6070, JEOL)



  P1-09: Funduscopy( 19w ) sop_b.png  

 
  Mouse retinal morphology is observed through the funduscope camera.
  Retina, blood vessels,and optic disc are observed in diluted eye.


  P1-10: X-ray ( 22w ) sop_b.png  


  Bone morphology is observed, using x-ray systems for specimen radiography.



  P1-11: Body fat percentage and Bone Mineral Density(DEXA)( 22w )
   sop_b.png   sop_b.png

  The Body fat percentage (%Fat) and Bone Mineral Density (BMD) are measured
  simultaneously by DEXA method (dual-energy x-ray absorptiometry)
  Analysis by PIXImus2 (LUNAR)


  P1-12:「 ECG (Electrocardiography)」( 24w )

p1-17_g3.png(57639 byte)

  To analyse cardiac functions, electrocardiogram (ECG) is recorded
using a system with surface electrodes that monitor patterns of potential changes within mice originated from the cardiac activity.


  P1-13: FACS ( 26w ) sop_b.png  

  This test differentiates immune cell sub-populations via flow cytometry.
 
  Measurement the mouse splenic cell by BD LSRFortessa
  Analysis of mouse T cells by FlowJo software

Parameters



TOP