Japan Mouse Clinic

Japanese
Clinic inspection (pipeline01)

Pipeline 1 Comprehensive test items examining metabolic, blood/hematic, osseous/skeletal, cancer, cardiovascular, sensory, skin/fur, and neural/behavioral characteristics, construct pipeline 1.

P1-01: Modified-SHIRPA ( 8w )

The modified SHIRPA is a simple method for evaluating the characteristic behaviors and morphologies of mice. 56 items about various mouse behaviors and morphologies, and its sensory responses are tested by visual inspection and manual handling using simple equipments.

P1-02: Hematological test ( 9w )
Blood Collection by retroorbital puncture (Using EDTA for anti-coagulant)	In hematological test, 22 parameters of blood count (white blood cell count, red blood cell count, platelet, etc) are measured with about 200 micro-L of mouse blood.

Analysis by automatic hematology analyzer (XN-1000V, sysmex)

P1-03: Clinical biochemical test ( 11w )
Blood Collection by retro-orbital puncture (Using Heparin-Lithium for anti-coagulant) Centrifuge for serum separation (Use blood clot for HbA1c measurement) Analysis by automatic clinical biochemical analyzer (JCA-BM6070, JEOL)
In clinical biochemical test, 21 parameters of serum biochemical tests (LDH, AST, Glu, T-CHO, etc) are measured with mouse serum.

P1-04: IPGTT (Intra-peritoneal glucose tolerance test) ( 14w )
GLUCOCARD (ARKRAY, Inc)	The glucose tolerance test measures the clearance of a standardized glucose load from the body. Mice are fasted for approximately 16 hours (overnight), before a glucose solution is administered intraperitoneally. Subsequent blood glucose is measured at different time points during the following 2 hours using a portable blood glucose monitor (GLUCOCARD; ARKRAY Inc.).

P1-05: Adipocytokine test ( 18w )
Blood Collection by retro-orbital puncture	adipocytokine
Centrifuge for serum separation
Analysis by multiplex suspension array system (BioPlex, Bio-Rad)

P1-06: Funduscopy ( 19w )

Mouse retinal morphology is observed through the funduscope camera. Retina, blood vessels,and optic disc are observed in diluted eye.

P1-07: X-ray ( 22w )

	Bone morphology is observed, using x-ray systems for specimen radiography.

P1-08: Body fat percentage and Bone Mineral Density (DEXA) ( 22w )
The Body fat percentage (%Fat) and Bone Mineral Density (BMD) are measured simultaneously by DEXA method (dual-energy x-ray absorptiometry)

Analysis by PIXImus2 (LUNAR)

P1-09:ECG (Electrocardiography) ( 24w )

To analyse cardiac functions, electrocardiogram (ECG) is recorded using a system with surface electrodes that monitor patterns of potential changes within mice originated from the cardiac activity.

P1-10: FACS ( 26w )
This test differentiates immune cell sub-populations via flow cytometry.

Measurement the mouse splenic cell by BD LSRFortessa	Analysis of mouse T cells by FlowJo software

Parameters

P1-11: Macroscopic test ( 26w )

After the early-onset phenotype analyses of mice in 13 weeks after birth, pathological phenotype analyses were commenced and to be continued until 26weeks of age. During this period, anatomical and histological test (option) .

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