Procedure Intraperitoneal glucose tolerance test (IPGTT)
Pipeline IMPC_adult_v2-LaterOnset Screen Age (week) 13-53w
Parameter Blood glucose concentration
Strain Condition 1 Pipeline(s) Tested Day
(Age)
IMPC_adult_v2-LaterOnset
C57BL/6NJcl Late ♀: 7  ♂: 7 2015/11/05 - 2016/08/09
(13.64 - 53.93)
Early ♀: 14  ♂: 13
Raw Data

Conditions of statistics.

sex Female

Bartlett test result
Factor df Bartlett's K2 p
condition1 1 2.107388 0.1465893
within 4 46.32843 2.104171e-09

Mauchly's test of sphericity
Factor Mauchly's W p

Summary statistics
Factor mean median SD SEM N
condition1 - within
Early(13w) - 0 60.29 58 10.77 2.88 14
Early(13w) - 15 332.93 307.5 90.17 24.1 14
Early(13w) - 30 283 283.5 77.08 20.6 14
Early(13w) - 60 186.43 198 30.35 8.11 14
Early(13w) - 120 87.5 84.5 14.78 3.95 14
Late(53w) - 0 89.43 84 22.23 8.4 7
Late(53w) - 15 308 291 66.19 25.02 7
Late(53w) - 30 262.57 279 78.34 29.61 7
Late(53w) - 60 201.43 207 37.72 14.26 7
Late(53w) - 120 138.43 127 52.52 19.85 7

ANOVA result
Signif. codes ***: <0.001
**: <0.010
*: <0.050
†: <0.100
Factor df Sum Sq. Mean Sq. F value Pr(>F) Signif.
condition1 1 2307 2307 0.3217 0.57726
Residuals 19 136260 7172
within 4 991443 247861 122.2071 2e-16 ***
condition1:within 4 19658 4915 2.4231 0.05536
Residuals 76 154143 2028

Pairwise comparisons using t tests with pooled SD
P value adjust method : Bonferroni

Factor: within
Pair p
0 - 15 0
0 - 30 0
0 - 60 0
15 - 60 0
15 - 120 0
30 - 60 0
30 - 120 0
60 - 120 0

Conditions of statistics.

sex Male

Bartlett test result
Factor df Bartlett's K2 p
condition1 1 1.843646 0.1745243
within 4 44.67693 4.640888e-09

Mauchly's test of sphericity
Factor Mauchly's W p

Summary statistics
Factor mean median SD SEM N
condition1 - within
Early(13w) - 0 67.92 67 10.14 2.81 13
Early(13w) - 15 323.92 325 56.65 15.71 13
Early(13w) - 30 380.54 386 56.08 15.55 13
Early(13w) - 60 313.23 307 58.68 16.28 13
Early(13w) - 120 154.15 152 33.52 9.3 13
Late(53w) - 0 98.14 97 8.73 3.3 7
Late(53w) - 15 297.86 298 80.95 30.6 7
Late(53w) - 30 279.29 240 87.7 33.15 7
Late(53w) - 60 261.57 217 104.11 39.35 7
Late(53w) - 120 172.57 191 38.34 14.49 7

ANOVA result
Signif. codes ***: <0.001
**: <0.010
*: <0.050
†: <0.100
Factor df Sum Sq. Mean Sq. F value Pr(>F) Signif.
condition1 1 15460 15460 1.6104 0.2206
Residuals 18 172795 9600
within 4 1041247 260312 157.1345 2.2e-16 ***
condition1:within 4 52120 13030 7.8654 2.565e-05 ***
Residuals 72 119276 1657

Pairwise comparisons using t tests with pooled SD
P value adjust method : Bonferroni

Factor: within
Pair p
0 - 15 0
0 - 30 0
0 - 60 0
0 - 120 6e-04
15 - 120 0
30 - 120 0
60 - 120 0

Factor: condition1:within
Pair p
Early(13w):0 - Early(13w):120 0.0096
Early(13w):0 - Early(13w):15 0
Early(13w):0 - Early(13w):30 0
Early(13w):0 - Early(13w):60 0
Early(13w):0 - Late(53w):120 0.0078
Early(13w):0 - Late(53w):15 0
Early(13w):0 - Late(53w):30 0
Early(13w):0 - Late(53w):60 0
Early(13w):120 - Early(13w):15 0
Early(13w):120 - Early(13w):30 0
Early(13w):120 - Early(13w):60 0
Early(13w):120 - Late(53w):15 0
Early(13w):120 - Late(53w):30 4e-04
Early(13w):120 - Late(53w):60 0.0054
Early(13w):15 - Late(53w):0 0
Early(13w):15 - Late(53w):120 0
Early(13w):30 - Late(53w):0 0
Early(13w):30 - Late(53w):120 0
Early(13w):30 - Late(53w):30 0.0122
Early(13w):30 - Late(53w):60 0.0011
Early(13w):60 - Late(53w):0 0
Early(13w):60 - Late(53w):120 0
Late(53w):0 - Late(53w):15 0
Late(53w):0 - Late(53w):30 0
Late(53w):0 - Late(53w):60 0
Late(53w):120 - Late(53w):15 0.0039
Late(53w):120 - Late(53w):30 0.0322



F_02_U_errorbar.png M_02_U_errorbar.png

comment
Blood glucose concentration (30 min) was significantly decreased in 53-week-old mice (Late) relative to 13-week-old mice (Early) (p<0.05) in only male.