Procedure Indirect Calorimetry v1
Pipeline IMPC_adult_v2-LaterOnset Screen Age (week) 11-51w
Parameter Total activity (no. of fine movement + no.of beam cuts)
Strain Condition 1 Pipeline(s) Tested Day
(Age)
IMPC_adult_v2-LaterOnset
C57BL/6NJcl Late ♀: 8  ♂: 8 2015/10/20 - 2016/07/26
(11.5 - 51.93)
Early ♀: 8  ♂: 8
Raw Data

Conditions of statistics.

sex Female

Bartlett test result
Factor df Bartlett's K2 p
condition1 1 46.16121 1.089118e-11
within 20 176.2883 5.159653e-27

Mauchly's test of sphericity
Factor Mauchly's W p

Summary statistics
Factor mean median SD SEM N
condition1 - within
Early(11w) - 1 245.62 111.5 258.54 91.41 8
Early(11w) - 2 141.75 47.5 232.83 82.32 8
Early(11w) - 3 187.5 216.5 140.91 49.82 8
Early(11w) - 4 294.75 143.5 329.73 116.58 8
Early(11w) - 5 405.62 363.5 347.78 122.96 8
Early(11w) - 6 475.25 449.5 184.97 65.4 8
Early(11w) - 7 1457.88 1300 660 233.35 8
Early(11w) - 8 2049 1923 1007.34 356.15 8
Early(11w) - 9 1902.88 1905 1009.28 356.83 8
Early(11w) - 10 1892.75 2057.5 787.25 278.34 8
Early(11w) - 11 1850.12 1622.5 835.46 295.38 8
Early(11w) - 12 1202.12 867.5 706.94 249.94 8
Early(11w) - 13 504.62 493 492.91 174.27 8
Early(11w) - 14 356.62 227 402.05 142.15 8
Early(11w) - 15 543.75 407 525.93 185.95 8
Early(11w) - 16 1160.5 1139 644.91 228.01 8
Early(11w) - 17 1184.25 910.5 801.89 283.51 8
Early(11w) - 18 960.38 817.5 541.05 191.29 8
Early(11w) - 19 290.88 205 317.51 112.26 8
Early(11w) - 20 526.5 546 388.38 137.31 8
Early(11w) - 21 106.38 79.5 113.9 40.27 8
Late(51w) - 1 237.25 180.5 304.03 107.49 8
Late(51w) - 2 216.12 71.5 306.25 108.28 8
Late(51w) - 3 233.38 152 243.53 86.1 8
Late(51w) - 4 298 120.5 457.09 161.61 8
Late(51w) - 5 343.75 285.5 279.98 98.99 8
Late(51w) - 6 311.62 190 255.17 90.22 8
Late(51w) - 7 812 626.5 606.59 214.46 8
Late(51w) - 8 1029.38 727 863.83 305.41 8
Late(51w) - 9 924.5 802.5 787.82 278.54 8
Late(51w) - 10 638.62 359 900.49 318.37 8
Late(51w) - 11 364.12 141.5 506.55 179.09 8
Late(51w) - 12 538.25 408.5 452.51 159.99 8
Late(51w) - 13 373.75 254.5 281.79 99.63 8
Late(51w) - 14 270.5 159 282.39 99.84 8
Late(51w) - 15 274.62 170 237.12 83.84 8
Late(51w) - 16 365.38 250.5 395.81 139.94 8
Late(51w) - 17 633.25 483.5 375.57 132.78 8
Late(51w) - 18 361.5 251 362.18 128.05 8
Late(51w) - 19 314.38 260 253.45 89.61 8
Late(51w) - 20 194.5 148.5 164.23 58.06 8
Late(51w) - 21 196.62 89 259.2 91.64 8

ANOVA result
Signif. codes ***: <0.001
**: <0.010
*: <0.050
†: <0.100
Factor df Sum Sq. Mean Sq. F value Pr(>F) Signif.
condition1 1 14776049 14776049 10.0380 0.006839 **
Residuals 14 20608213 1472015
within 20 61407675 3070384 14.5302 2.2e-16 ***
condition1:within 20 17961762 898088 4.2501 1.500e-08 ***
Residuals 280 59167117 211311

Pairwise comparisons using t tests with pooled SD
P value adjust method : Bonferroni

Factor: condition1
Pair p
Early(11w) - Late(51w) 0

Factor: within
Pair p
1 - 7 0.0065
1 - 8 0
1 - 9 0
1 - 10 4e-04
1 - 11 0.0112
2 - 7 0.0018
2 - 8 0
2 - 9 0
2 - 10 1e-04
2 - 11 0.0032
3 - 7 0.0035
3 - 8 0
3 - 9 0
3 - 10 2e-04
3 - 11 0.0061
4 - 7 0.0188
4 - 8 0
4 - 9 0
4 - 10 0.0014
4 - 11 0.0316
5 - 8 0
5 - 9 3e-04
5 - 10 0.0068
6 - 8 0
6 - 9 5e-04
6 - 10 0.0099
7 - 14 0.026
7 - 19 0.0212
7 - 21 0.001
8 - 13 1e-04
8 - 14 0
8 - 15 0
8 - 18 0.0088
8 - 19 0
8 - 20 0
8 - 21 0
9 - 13 0.0012
9 - 14 1e-04
9 - 15 6e-04
9 - 19 1e-04
9 - 20 2e-04
9 - 21 0
10 - 13 0.0236
10 - 14 0.002
10 - 15 0.0134
10 - 19 0.0016
10 - 20 0.0051
10 - 21 1e-04
11 - 14 0.0432
11 - 19 0.0354
11 - 21 0.0018

Factor: condition1:within
Pair p
Early(11w):1 - Early(11w):10 0
Early(11w):1 - Early(11w):11 0
Early(11w):1 - Early(11w):7 0.0042
Early(11w):1 - Early(11w):8 0
Early(11w):1 - Early(11w):9 0
Early(11w):10 - Early(11w):13 2e-04
Early(11w):10 - Early(11w):14 0
Early(11w):10 - Early(11w):15 4e-04
Early(11w):10 - Early(11w):19 0
Early(11w):10 - Early(11w):2 0
Early(11w):10 - Early(11w):20 3e-04
Early(11w):10 - Early(11w):21 0
Early(11w):10 - Early(11w):3 0
Early(11w):10 - Early(11w):4 0
Early(11w):10 - Early(11w):5 0
Early(11w):10 - Early(11w):6 1e-04
Early(11w):10 - Late(51w):1 0
Early(11w):10 - Late(51w):10 0.002
Early(11w):10 - Late(51w):11 0
Early(11w):10 - Late(51w):12 3e-04
Early(11w):10 - Late(51w):13 0
Early(11w):10 - Late(51w):14 0
Early(11w):10 - Late(51w):15 0
Early(11w):10 - Late(51w):16 0
Early(11w):10 - Late(51w):17 0.0018
Early(11w):10 - Late(51w):18 0
Early(11w):10 - Late(51w):19 0
Early(11w):10 - Late(51w):2 0
Early(11w):10 - Late(51w):20 0
Early(11w):10 - Late(51w):21 0
Early(11w):10 - Late(51w):3 0
Early(11w):10 - Late(51w):4 0
Early(11w):10 - Late(51w):5 0
Early(11w):10 - Late(51w):6 0
Early(11w):10 - Late(51w):7 0.0376
Early(11w):11 - Early(11w):13 4e-04
Early(11w):11 - Early(11w):14 0
Early(11w):11 - Early(11w):15 8e-04
Early(11w):11 - Early(11w):19 0
Early(11w):11 - Early(11w):2 0
Early(11w):11 - Early(11w):20 6e-04
Early(11w):11 - Early(11w):21 0
Early(11w):11 - Early(11w):3 0
Early(11w):11 - Early(11w):4 0
Early(11w):11 - Early(11w):5 1e-04
Early(11w):11 - Early(11w):6 2e-04
Early(11w):11 - Late(51w):1 0
Early(11w):11 - Late(51w):10 0.0043
Early(11w):11 - Late(51w):11 0
Early(11w):11 - Late(51w):12 7e-04
Early(11w):11 - Late(51w):13 0
Early(11w):11 - Late(51w):14 0
Early(11w):11 - Late(51w):15 0
Early(11w):11 - Late(51w):16 0
Early(11w):11 - Late(51w):17 0.0039
Early(11w):11 - Late(51w):18 0
Early(11w):11 - Late(51w):19 0
Early(11w):11 - Late(51w):2 0
Early(11w):11 - Late(51w):20 0
Early(11w):11 - Late(51w):21 0
Early(11w):11 - Late(51w):3 0
Early(11w):11 - Late(51w):4 0
Early(11w):11 - Late(51w):5 0
Early(11w):11 - Late(51w):6 0
Early(11w):12 - Early(11w):21 0.0296
Early(11w):13 - Early(11w):8 0
Early(11w):13 - Early(11w):9 1e-04
Early(11w):14 - Early(11w):7 0.0271
Early(11w):14 - Early(11w):8 0
Early(11w):14 - Early(11w):9 0
Early(11w):15 - Early(11w):8 0
Early(11w):15 - Early(11w):9 3e-04
Early(11w):17 - Early(11w):21 0.0394
Early(11w):18 - Early(11w):8 0.0332
Early(11w):19 - Early(11w):7 0.0092
Early(11w):19 - Early(11w):8 0
Early(11w):19 - Early(11w):9 0
Early(11w):2 - Early(11w):7 7e-04
Early(11w):2 - Early(11w):8 0
Early(11w):2 - Early(11w):9 0
Early(11w):20 - Early(11w):8 0
Early(11w):20 - Early(11w):9 2e-04
Early(11w):21 - Early(11w):7 3e-04
Early(11w):21 - Early(11w):8 0
Early(11w):21 - Early(11w):9 0
Early(11w):3 - Early(11w):7 0.0015
Early(11w):3 - Early(11w):8 0
Early(11w):3 - Early(11w):9 0
Early(11w):4 - Early(11w):7 0.0098
Early(11w):4 - Early(11w):8 0
Early(11w):4 - Early(11w):9 0
Early(11w):5 - Early(11w):8 0
Early(11w):5 - Early(11w):9 0
Early(11w):6 - Early(11w):8 0
Early(11w):6 - Early(11w):9 1e-04
Early(11w):7 - Late(51w):1 0.0037
Early(11w):7 - Late(51w):11 0.0306
Early(11w):7 - Late(51w):13 0.0356
Early(11w):7 - Late(51w):14 0.0065
Early(11w):7 - Late(51w):15 0.007
Early(11w):7 - Late(51w):16 0.0312
Early(11w):7 - Late(51w):18 0.0293
Early(11w):7 - Late(51w):19 0.0136
Early(11w):7 - Late(51w):2 0.0025
Early(11w):7 - Late(51w):20 0.0017
Early(11w):7 - Late(51w):21 0.0018
Early(11w):7 - Late(51w):3 0.0034
Early(11w):7 - Late(51w):4 0.0104
Early(11w):7 - Late(51w):5 0.022
Early(11w):7 - Late(51w):6 0.013
Early(11w):8 - Late(51w):1 0
Early(11w):8 - Late(51w):10 1e-04
Early(11w):8 - Late(51w):11 0
Early(11w):8 - Late(51w):12 0
Early(11w):8 - Late(51w):13 0
Early(11w):8 - Late(51w):14 0
Early(11w):8 - Late(51w):15 0
Early(11w):8 - Late(51w):16 0
Early(11w):8 - Late(51w):17 1e-04
Early(11w):8 - Late(51w):18 0
Early(11w):8 - Late(51w):19 0
Early(11w):8 - Late(51w):2 0
Early(11w):8 - Late(51w):20 0
Early(11w):8 - Late(51w):21 0
Early(11w):8 - Late(51w):3 0
Early(11w):8 - Late(51w):4 0
Early(11w):8 - Late(51w):5 0
Early(11w):8 - Late(51w):6 0
Early(11w):8 - Late(51w):7 0.0027
Early(11w):8 - Late(51w):9 0.0186
Early(11w):9 - Late(51w):1 0
Early(11w):9 - Late(51w):10 0.0017
Early(11w):9 - Late(51w):11 0
Early(11w):9 - Late(51w):12 3e-04
Early(11w):9 - Late(51w):13 0
Early(11w):9 - Late(51w):14 0
Early(11w):9 - Late(51w):15 0
Early(11w):9 - Late(51w):16 0
Early(11w):9 - Late(51w):17 0.0015
Early(11w):9 - Late(51w):18 0
Early(11w):9 - Late(51w):19 0
Early(11w):9 - Late(51w):2 0
Early(11w):9 - Late(51w):20 0
Early(11w):9 - Late(51w):21 0
Early(11w):9 - Late(51w):3 0
Early(11w):9 - Late(51w):4 0
Early(11w):9 - Late(51w):5 0
Early(11w):9 - Late(51w):6 0
Early(11w):9 - Late(51w):7 0.032

Conditions of statistics.

sex Male

Bartlett test result
Factor df Bartlett's K2 p
condition1 1 53.48064 2.611544e-13
within 20 103.9634 2.443959e-13

Mauchly's test of sphericity
Factor Mauchly's W p

Summary statistics
Factor mean median SD SEM N
condition1 - within
Early(11w) - 1 119.62 63.5 143.3 50.67 8
Early(11w) - 2 156.62 56 248.3 87.79 8
Early(11w) - 3 113.62 40.5 184.47 65.22 8
Early(11w) - 4 130 76.5 118.88 42.03 8
Early(11w) - 5 67 44 50.02 17.69 8
Early(11w) - 6 465.62 487.5 273.24 96.61 8
Early(11w) - 7 882.25 923.5 448.85 158.69 8
Early(11w) - 8 706.75 750 414.95 146.71 8
Early(11w) - 9 448.75 405.5 266.03 94.05 8
Early(11w) - 10 416.38 353.5 262.63 92.85 8
Early(11w) - 11 448.5 363.5 347.33 122.8 8
Early(11w) - 12 431.62 458 298.4 105.5 8
Early(11w) - 13 440.62 433 259.05 91.59 8
Early(11w) - 14 206.75 123 228.3 80.72 8
Early(11w) - 15 159.75 131 122.81 43.42 8
Early(11w) - 16 688.38 607 362.29 128.09 8
Early(11w) - 17 491.25 429 232.58 82.23 8
Early(11w) - 18 563.5 415.5 564.57 199.6 8
Early(11w) - 19 69.12 66.5 55.5 19.62 8
Early(11w) - 20 163.62 138.5 133.39 47.16 8
Early(11w) - 21 250.5 205.5 254.72 90.06 8
Late(51w) - 1 131.75 71.5 155.13 54.85 8
Late(51w) - 2 112.38 105 102.83 36.36 8
Late(51w) - 3 157.88 29.5 207.46 73.35 8
Late(51w) - 4 104 70.5 114.58 40.51 8
Late(51w) - 5 195.75 151 199.35 70.48 8
Late(51w) - 6 210.75 167.5 178.23 63.01 8
Late(51w) - 7 534.38 482.5 323.26 114.29 8
Late(51w) - 8 236.38 145.5 217.76 76.99 8
Late(51w) - 9 328.25 246 265.33 93.81 8
Late(51w) - 10 221.88 236 133.65 47.25 8
Late(51w) - 11 146.25 109 130.65 46.19 8
Late(51w) - 12 110.12 59.5 183.06 64.72 8
Late(51w) - 13 114.12 120 96.46 34.1 8
Late(51w) - 14 263 190 265.54 93.88 8
Late(51w) - 15 216 169 147.68 52.21 8
Late(51w) - 16 216.38 211.5 138.29 48.89 8
Late(51w) - 17 379.62 394 146.31 51.73 8
Late(51w) - 18 249 215 192.57 68.08 8
Late(51w) - 19 181.25 161 121.91 43.1 8
Late(51w) - 20 157 192 89.35 31.59 8
Late(51w) - 21 158.88 156.5 101.2 35.78 8

ANOVA result
Signif. codes ***: <0.001
**: <0.010
*: <0.050
†: <0.100
Factor df Sum Sq. Mean Sq. F value Pr(>F) Signif.
condition1 1 1708861 1708861 11.9515 0.00385 **
Residuals 14 2001758 142983
within 20 7614263 380713 7.4864 2.2e-16 ***
condition1:within 20 2865980 143299 2.8178 8.232e-05 ***
Residuals 280 14239146 50854

Pairwise comparisons using t tests with pooled SD
P value adjust method : Bonferroni

Factor: condition1
Pair p
Early(11w) - Late(51w) 0

Factor: within
Pair p
1 - 7 0
1 - 8 0.0357
2 - 7 0
3 - 7 0
4 - 7 0
4 - 8 0.0246
5 - 7 0
5 - 8 0.0454
6 - 7 0.0124
7 - 10 0.0052
7 - 11 0.0018
7 - 12 5e-04
7 - 13 7e-04
7 - 14 1e-04
7 - 15 0
7 - 19 0
7 - 20 0
7 - 21 0
8 - 19 0.0349

Factor: condition1:within
Pair p
Early(11w):1 - Early(11w):16 0.0018
Early(11w):1 - Early(11w):7 0
Early(11w):1 - Early(11w):8 9e-04
Early(11w):14 - Early(11w):16 0.0464
Early(11w):14 - Early(11w):7 0
Early(11w):14 - Early(11w):8 0.0243
Early(11w):15 - Early(11w):16 0.0085
Early(11w):15 - Early(11w):7 0
Early(11w):15 - Early(11w):8 0.0042
Early(11w):16 - Early(11w):19 2e-04
Early(11w):16 - Early(11w):2 0.0076
Early(11w):16 - Early(11w):20 0.0098
Early(11w):16 - Early(11w):3 0.0014
Early(11w):16 - Early(11w):4 0.0027
Early(11w):16 - Early(11w):5 2e-04
Early(11w):16 - Late(51w):1 0.0029
Early(11w):16 - Late(51w):11 0.0051
Early(11w):16 - Late(51w):12 0.0012
Early(11w):16 - Late(51w):13 0.0015
Early(11w):16 - Late(51w):19 0.0188
Early(11w):16 - Late(51w):2 0.0014
Early(11w):16 - Late(51w):20 0.0077
Early(11w):16 - Late(51w):21 0.0082
Early(11w):16 - Late(51w):3 0.0079
Early(11w):16 - Late(51w):4 0.001
Early(11w):16 - Late(51w):5 0.0315
Early(11w):18 - Early(11w):19 0.0296
Early(11w):18 - Early(11w):5 0.0275
Early(11w):19 - Early(11w):7 0
Early(11w):19 - Early(11w):8 1e-04
Early(11w):2 - Early(11w):7 0
Early(11w):2 - Early(11w):8 0.0038
Early(11w):20 - Early(11w):7 0
Early(11w):20 - Early(11w):8 0.0049
Early(11w):21 - Early(11w):7 1e-04
Early(11w):3 - Early(11w):7 0
Early(11w):3 - Early(11w):8 7e-04
Early(11w):4 - Early(11w):7 0
Early(11w):4 - Early(11w):8 0.0013
Early(11w):5 - Early(11w):7 0
Early(11w):5 - Early(11w):8 1e-04
Early(11w):7 - Late(51w):1 0
Early(11w):7 - Late(51w):10 0
Early(11w):7 - Late(51w):11 0
Early(11w):7 - Late(51w):12 0
Early(11w):7 - Late(51w):13 0
Early(11w):7 - Late(51w):14 2e-04
Early(11w):7 - Late(51w):15 0
Early(11w):7 - Late(51w):16 0
Early(11w):7 - Late(51w):17 0.0221
Early(11w):7 - Late(51w):18 1e-04
Early(11w):7 - Late(51w):19 0
Early(11w):7 - Late(51w):2 0
Early(11w):7 - Late(51w):20 0
Early(11w):7 - Late(51w):21 0
Early(11w):7 - Late(51w):3 0
Early(11w):7 - Late(51w):4 0
Early(11w):7 - Late(51w):5 0
Early(11w):7 - Late(51w):6 0
Early(11w):7 - Late(51w):8 1e-04
Early(11w):7 - Late(51w):9 0.0032
Early(11w):8 - Late(51w):1 0.0014
Early(11w):8 - Late(51w):10 0.0414
Early(11w):8 - Late(51w):11 0.0025
Early(11w):8 - Late(51w):12 6e-04
Early(11w):8 - Late(51w):13 7e-04
Early(11w):8 - Late(51w):15 0.0337
Early(11w):8 - Late(51w):16 0.0341
Early(11w):8 - Late(51w):19 0.0096
Early(11w):8 - Late(51w):2 6e-04
Early(11w):8 - Late(51w):20 0.0038
Early(11w):8 - Late(51w):21 0.0041
Early(11w):8 - Late(51w):3 0.0039
Early(11w):8 - Late(51w):4 5e-04
Early(11w):8 - Late(51w):5 0.0163
Early(11w):8 - Late(51w):6 0.028



F_02_U_errorbar.png M_02_U_errorbar.png

comment
Total activity (no. of fine movement + no.of beam cuts) was significantly decreased in 51-week-old mice (Late) relative to 11-week-old mice (Early) in both sexs (p<0.01).