Genotype Viewer

Analysis Group	Condition; Aged mice (B6/NJcl)
Analysis Group Description	Phenotypic differences of C57BL/6NJcl strain between early and late age have been analyzed in this analysis group. Test mice were produced by the natural mating and reared under the JMC standard housing conditions.

Procedure	Indirect Calorimetry v1
Pipeline	IMPC_adult_v2-LaterOnset	Screen Age (week)	11-51w
Parameter	Heat production (metaboric rate)

Strain	Associated Gene(s)	Housing SOP(s) and SDOP(s)
	C57BL/6NJcl		Mouse husbandry (RIKEN BRC)

Strain	C57BL/6NJcl	Strain description
Associated Gene(s)		This strain was provided from CLEA Japan and has been maintained by natural mating for two generations.
Housing SOP(s) and SDOP(s)	Mouse husbandry (RIKEN BRC)
Tested Day (Age)	2015/10/20 - 2016/07/26 (11.5 - 51.93)

Strain	Condition 1	Pipeline(s)	Tested Day (Age)
IMPC_adult_v2-LaterOnset
C57BL/6NJcl	Late	♀: 8 ♂: 8	2015/10/20 - 2016/07/26 (11.5 - 51.93)
Early	♀: 8 ♂: 8

sex	Female

Bartlett test result
Factor	df	Bartlett's K²	p
condition1	1	1.262601	0.2611593
within	20	18.14534	0.5778347

Mauchly's test of sphericity
Factor	Mauchly's W	p

Summary statistics
Factor	mean	median	SD	SEM	N
condition1 - within	mean	median	SD	SEM	N
Early(11w) - 1	1.57	1.57	0.3	0.11	8
Early(11w) - 2	1.65	1.59	0.27	0.09	8
Early(11w) - 3	1.63	1.52	0.31	0.11	8
Early(11w) - 4	1.88	1.76	0.6	0.21	8
Early(11w) - 5	1.83	1.75	0.37	0.13	8
Early(11w) - 6	1.91	1.8	0.5	0.18	8
Early(11w) - 7	2.14	2.17	0.29	0.1	8
Early(11w) - 8	2.32	2.43	0.48	0.17	8
Early(11w) - 9	2.18	2.28	0.44	0.16	8
Early(11w) - 10	2.54	2.51	0.41	0.14	8
Early(11w) - 11	2.13	2.33	0.48	0.17	8
Early(11w) - 12	2.11	2.07	0.25	0.09	8
Early(11w) - 13	1.81	1.79	0.39	0.14	8
Early(11w) - 14	1.74	1.68	0.33	0.12	8
Early(11w) - 15	1.73	1.68	0.25	0.09	8
Early(11w) - 16	1.87	1.79	0.44	0.16	8
Early(11w) - 17	2.09	2.02	0.29	0.1	8
Early(11w) - 18	1.88	1.82	0.52	0.18	8
Early(11w) - 19	1.64	1.62	0.39	0.14	8
Early(11w) - 20	1.78	1.81	0.39	0.14	8
Early(11w) - 21	1.7	1.66	0.21	0.07	8
Late(51w) - 1	2.08	2	0.29	0.1	8
Late(51w) - 2	2.01	2.04	0.47	0.17	8
Late(51w) - 3	2.24	2.08	0.42	0.15	8
Late(51w) - 4	2.1	2.21	0.34	0.12	8
Late(51w) - 5	2.2	2.23	0.18	0.06	8
Late(51w) - 6	2.35	2.23	0.38	0.14	8
Late(51w) - 7	2.36	2.42	0.28	0.1	8
Late(51w) - 8	2.59	2.8	0.6	0.21	8
Late(51w) - 9	2.74	2.84	0.48	0.17	8
Late(51w) - 10	2.41	2.36	0.61	0.22	8
Late(51w) - 11	2.41	2.25	0.73	0.26	8
Late(51w) - 12	2.69	2.62	0.57	0.2	8
Late(51w) - 13	2.2	2.17	0.41	0.15	8
Late(51w) - 14	2.35	2.37	0.38	0.13	8
Late(51w) - 15	2.11	2.11	0.37	0.13	8
Late(51w) - 16	2.15	2.09	0.31	0.11	8
Late(51w) - 17	2.32	2.5	0.62	0.22	8
Late(51w) - 18	2.36	2.36	0.57	0.2	8
Late(51w) - 19	2.24	2.3	0.56	0.2	8
Late(51w) - 20	2.13	2.1	0.37	0.13	8
Late(51w) - 21	2.18	2.16	0.54	0.19	8

ANOVA result
Factor	df	Sum Sq.	Mean Sq.	F value	Pr(>F)	Signif.
Signif. codes *: <0.001 : <0.010 *: <0.050 †: <0.100
condition1	1	12.496	12.496	13.7278	0.002353	**
Residuals	14	12.744	0.910
within	20	13.874	0.694	4.6035	1.733e-09	***
condition1:within	20	2.539	0.127	0.8425	0.660528
Residuals	280	42.193	0.151

Factor: condition1
Pair	p
Early(11w) - Late(51w)	0

sex	Male

Bartlett test result
Factor	df	Bartlett's K²	p
condition1	1	0.08181947	0.7748467
within	20	33.02782	0.03350433

Mauchly's test of sphericity
Factor	Mauchly's W	p

Summary statistics
Factor	mean	median	SD	SEM	N
condition1 - within	mean	median	SD	SEM	N
Early(11w) - 1	1.92	1.84	0.31	0.11	8
Early(11w) - 2	1.84	1.79	0.31	0.11	8
Early(11w) - 3	1.82	1.87	0.23	0.08	8
Early(11w) - 4	1.79	1.71	0.19	0.07	8
Early(11w) - 5	2.03	2.09	0.36	0.13	8
Early(11w) - 6	2.11	2.05	0.28	0.1	8
Early(11w) - 7	2.49	2.45	0.4	0.14	8
Early(11w) - 8	2.81	2.64	0.62	0.22	8
Early(11w) - 9	2.31	2.15	0.46	0.16	8
Early(11w) - 10	2.33	2.28	0.49	0.17	8
Early(11w) - 11	2.09	2.11	0.46	0.16	8
Early(11w) - 12	2.06	2.05	0.12	0.04	8
Early(11w) - 13	2.51	2.16	0.7	0.25	8
Early(11w) - 14	2.13	1.87	0.62	0.22	8
Early(11w) - 15	2.36	2.26	0.42	0.15	8
Early(11w) - 16	2.28	2.09	0.62	0.22	8
Early(11w) - 17	2.47	2.35	0.4	0.14	8
Early(11w) - 18	1.91	1.76	0.51	0.18	8
Early(11w) - 19	1.88	1.7	0.38	0.13	8
Early(11w) - 20	1.92	1.99	0.21	0.07	8
Early(11w) - 21	1.8	1.73	0.39	0.14	8
Late(51w) - 1	2.16	2.17	0.17	0.06	8
Late(51w) - 2	2.58	2.51	0.58	0.21	8
Late(51w) - 3	2.19	2.05	0.45	0.16	8
Late(51w) - 4	2.12	2.02	0.47	0.17	8
Late(51w) - 5	2.28	2.28	0.42	0.15	8
Late(51w) - 6	2.62	2.67	0.49	0.17	8
Late(51w) - 7	2.73	2.64	0.37	0.13	8
Late(51w) - 8	2.8	3.01	0.5	0.18	8
Late(51w) - 9	2.68	2.69	0.5	0.18	8
Late(51w) - 10	2.64	2.67	0.41	0.14	8
Late(51w) - 11	2.53	2.36	0.42	0.15	8
Late(51w) - 12	2.43	2.49	0.4	0.14	8
Late(51w) - 13	2.62	2.52	0.46	0.16	8
Late(51w) - 14	2.63	2.54	0.59	0.21	8
Late(51w) - 15	2.43	2.27	0.55	0.2	8
Late(51w) - 16	2.39	2.43	0.28	0.1	8
Late(51w) - 17	2.52	2.52	0.46	0.16	8
Late(51w) - 18	2.73	2.65	0.77	0.27	8
Late(51w) - 19	2.19	2.04	0.39	0.14	8
Late(51w) - 20	2.39	2.43	0.3	0.1	8
Late(51w) - 21	2.18	2.09	0.34	0.12	8

ANOVA result
Factor	df	Sum Sq.	Mean Sq.	F value	Pr(>F)	Signif.
Signif. codes *: <0.001 : <0.010 *: <0.050 †: <0.100
condition1	1	9.331	9.331	14.8705	0.001746	**
Residuals	14	8.785	0.628
within	20	16.796	0.840	4.7364	7.708e-10	***
condition1:within	20	3.584	0.179	1.0107	0.449402
Residuals	280	49.647	0.177

Factor: condition1
Pair	p
Early(11w) - Late(51w)	0

comment
Heat production (metaboric rate) was significantly increased in 51-week-old mice (Late) relative to 11-week-old mice (Early) in both sexs (p<0.01).