Procedure Indirect Calorimetry v1
Pipeline IMPC_adult_v2-LaterOnset Screen Age (week) 11-51w
Parameter Carbon dioxide production
Strain Condition 1 Pipeline(s) Tested Day
(Age)
IMPC_adult_v2-LaterOnset
C57BL/6NJcl Late ♀: 8  ♂: 8 2015/10/20 - 2016/07/26
(11.5 - 51.93)
Early ♀: 8  ♂: 8
Raw Data

Conditions of statistics.

sex Female

Bartlett test result
Factor df Bartlett's K2 p
condition1 1 0.8834973 0.3472456
within 20 21.55687 0.3650317

Mauchly's test of sphericity
Factor Mauchly's W p

Summary statistics
Factor mean median SD SEM N
condition1 - within
Early(11w) - 1 64.52 62.8 16.27 5.75 8
Early(11w) - 2 67.67 60.91 14.13 5 8
Early(11w) - 3 65.91 59.05 13.78 4.87 8
Early(11w) - 4 80.96 75.05 27.73 9.8 8
Early(11w) - 5 78.16 71.64 17.04 6.03 8
Early(11w) - 6 81.93 75.87 24.16 8.54 8
Early(11w) - 7 93.71 94.26 13.05 4.62 8
Early(11w) - 8 104.31 110.44 20.29 7.17 8
Early(11w) - 9 98.96 102.72 19.86 7.02 8
Early(11w) - 10 115.44 112.27 19.86 7.02 8
Early(11w) - 11 97.23 106.39 22.4 7.92 8
Early(11w) - 12 95.65 94.21 11.87 4.2 8
Early(11w) - 13 80.5 80.12 18.62 6.58 8
Early(11w) - 14 76.48 73.46 15.74 5.56 8
Early(11w) - 15 74.12 72.38 11.19 3.96 8
Early(11w) - 16 81.82 78.8 21.05 7.44 8
Early(11w) - 17 93.43 90.62 13.96 4.93 8
Early(11w) - 18 82.68 81.2 25.15 8.89 8
Early(11w) - 19 72.44 71.98 17.82 6.3 8
Early(11w) - 20 77.99 82.25 19.11 6.76 8
Early(11w) - 21 74.22 75.2 11.42 4.04 8
Late(51w) - 1 87.49 82.16 18.15 6.42 8
Late(51w) - 2 84.42 90.3 21.05 7.44 8
Late(51w) - 3 94.35 85.48 23.7 8.38 8
Late(51w) - 4 88.38 94.89 18.76 6.63 8
Late(51w) - 5 93.94 93.1 10.11 3.57 8
Late(51w) - 6 103.22 101.25 20.06 7.09 8
Late(51w) - 7 102.31 104.08 10.96 3.87 8
Late(51w) - 8 114.41 123.03 26.32 9.3 8
Late(51w) - 9 122.89 122.55 20.82 7.36 8
Late(51w) - 10 106.32 108.68 24.91 8.81 8
Late(51w) - 11 106.67 102.32 36.96 13.07 8
Late(51w) - 12 121.06 120.67 25.71 9.09 8
Late(51w) - 13 96.75 91.59 20.7 7.32 8
Late(51w) - 14 102.66 97.56 18.55 6.56 8
Late(51w) - 15 92.27 94.86 15.5 5.48 8
Late(51w) - 16 93.92 94.24 11.86 4.19 8
Late(51w) - 17 101.24 109.74 29.06 10.27 8
Late(51w) - 18 103.62 102.88 27.84 9.84 8
Late(51w) - 19 98.32 98.66 27.13 9.59 8
Late(51w) - 20 92.06 90.92 15.54 5.49 8
Late(51w) - 21 94.42 90.01 26.85 9.49 8

ANOVA result
Signif. codes ***: <0.001
**: <0.010
*: <0.050
†: <0.100
Factor df Sum Sq. Mean Sq. F value Pr(>F) Signif.
condition1 1 22352 22352 14.3287 0.002008 **
Residuals 14 21839 1560
within 20 39239 1962 5.3935 1.421e-11 ***
condition1:within 20 6209 310 0.8534 0.646808
Residuals 280 101855 364

Pairwise comparisons using t tests with pooled SD
P value adjust method : Bonferroni

Factor: condition1
Pair p
Early(11w) - Late(51w) 0

Factor: within
Pair p
1 - 8 0.005
1 - 9 0.0021
1 - 10 0.0021
1 - 12 0.0086
2 - 8 0.0051
2 - 9 0.0021
2 - 10 0.0022
2 - 12 0.0087
3 - 8 0.0424
3 - 9 0.0193
3 - 10 0.0198

Conditions of statistics.

sex Male

Bartlett test result
Factor df Bartlett's K2 p
condition1 1 2.480024 0.1153005
within 20 42.14772 0.002645548

Mauchly's test of sphericity
Factor Mauchly's W p

Summary statistics
Factor mean median SD SEM N
condition1 - within
Early(11w) - 1 80.76 76.98 14.53 5.14 8
Early(11w) - 2 76.37 75.63 14.93 5.28 8
Early(11w) - 3 76.43 76.44 12.62 4.46 8
Early(11w) - 4 76.38 74.2 11.17 3.95 8
Early(11w) - 5 88.47 92.33 18.94 6.69 8
Early(11w) - 6 93.74 89.42 14.39 5.09 8
Early(11w) - 7 111.47 110 18.04 6.38 8
Early(11w) - 8 129.18 122.16 28.9 10.22 8
Early(11w) - 9 106.84 100.53 20.41 7.21 8
Early(11w) - 10 107.12 104.69 22.81 8.06 8
Early(11w) - 11 96.23 97.85 22.02 7.79 8
Early(11w) - 12 94.77 94.38 5.12 1.81 8
Early(11w) - 13 113.73 98.54 31.59 11.17 8
Early(11w) - 14 97.19 88.77 28.36 10.03 8
Early(11w) - 15 107.81 102.62 20.49 7.24 8
Early(11w) - 16 103.67 95.37 28.5 10.08 8
Early(11w) - 17 112.15 105.53 19.66 6.95 8
Early(11w) - 18 86.6 79.18 24.19 8.55 8
Early(11w) - 19 85.33 76.25 18.69 6.61 8
Early(11w) - 20 85.83 87.55 9.43 3.33 8
Early(11w) - 21 76.49 73.13 21.07 7.45 8
Late(51w) - 1 93.06 89.63 10.47 3.7 8
Late(51w) - 2 113.67 118.17 26.35 9.32 8
Late(51w) - 3 96.73 86.98 23.7 8.38 8
Late(51w) - 4 91.44 92.71 19.68 6.96 8
Late(51w) - 5 99.51 98.84 18.93 6.69 8
Late(51w) - 6 119.98 117.71 27.83 9.84 8
Late(51w) - 7 125 117.32 23.95 8.47 8
Late(51w) - 8 126.4 135.51 22.36 7.9 8
Late(51w) - 9 123.68 119.44 31.44 11.11 8
Late(51w) - 10 121.74 120.33 22.71 8.03 8
Late(51w) - 11 114.82 113.57 19.92 7.04 8
Late(51w) - 12 109.48 108.78 21.25 7.51 8
Late(51w) - 13 121.13 110.38 34.28 12.12 8
Late(51w) - 14 121.12 112.28 35.65 12.6 8
Late(51w) - 15 110.45 98.68 34 12.02 8
Late(51w) - 16 108.94 107.5 18.13 6.41 8
Late(51w) - 17 115.59 110.25 25.77 9.11 8
Late(51w) - 18 127.04 118.39 51.65 18.26 8
Late(51w) - 19 98.86 99.34 18.5 6.54 8
Late(51w) - 20 109.53 108.78 22.36 7.91 8
Late(51w) - 21 96.5 92.09 16.29 5.76 8

ANOVA result
Signif. codes ***: <0.001
**: <0.010
*: <0.050
†: <0.100
Factor df Sum Sq. Mean Sq. F value Pr(>F) Signif.
condition1 1 21776 21776 6.1551 0.02642 *
Residuals 14 49530 3538
within 20 48438 2422 5.9124 6.251e-13 ***
condition1:within 20 9011 451 1.0999 0.34873
Residuals 280 114696 410

Pairwise comparisons using t tests with pooled SD
P value adjust method : Bonferroni

Factor: condition1
Pair p
Early(11w) - Late(51w) 0

Factor: within
Pair p
1 - 8 0.001
2 - 8 0.0486
3 - 8 9e-04
4 - 7 0.0245
4 - 8 2e-04
4 - 13 0.0349
5 - 8 0.0309
8 - 19 0.0131
8 - 21 8e-04



F_02_U_errorbar.png M_02_U_errorbar.png

comment
Carbon dioxide production was significantly increased in 51-week-old mice (Late) relative to 11-week-old mice (Early) in both sexs (Female: p<0.01, Male: P<0.05).