Procedure Indirect Calorimetry v1
Pipeline IMPC_adult_v2-LaterOnset Screen Age (week) 11-51w
Parameter Body weight before experiment
Strain Condition 1 Pipeline(s) Tested Day
(Age)
IMPC_adult_v2-LaterOnset
C57BL/6NJcl Late ♀: 8  ♂: 8 2015/10/20 - 2016/07/26
(11.5 - 51.93)
Early ♀: 8  ♂: 8
Raw Data

Bartlett test result
Factor df Bartlett's K2 p
condition1 1 11.91416 0.0005570891
sex 1 0.03067988 0.8609565

Summary statistics
Factor mean median SD SEM N
condition1 - sex
Early(11w) - Female 23.65 23.5 1.04 0.37 8
Early(11w) - Male 29.76 29.55 1.66 0.59 8
Late(51w) - Female 38.92 35.2 9.44 3.34 8
Late(51w) - Male 49.31 51.75 4.9 1.73 8

ANOVA result
Signif. codes ***: <0.001
**: <0.010
*: <0.050
†: <0.100
Factor df Sum Sq. F value Pr(>F) Signif.
condition1 1 2425.56 82.886 7.325e-10 ***
sex 1 544.50 18.607 0.0001804 ***
condition1:sex 1 36.55 1.249 0.2732354
Residuals 28 819.38

Tukey multiple comparisons of means
Factor: condition1
Level Difference Lower Upper Adjusted p
Late(51w) - Early(11w) 17.4 13.5 21.3 7.33e-10

Factor: sex
Level Difference Lower Upper Adjusted p
Male - Female 8.25 4.33 12.2 0.000180



FM_02_U_barchart.png

comment
Body weight before experiment was significantly increased in 51-week-old mice (Late) relative to 11-week-old mice (Early) (p<0.01).