Procedure Indirect Calorimetry v1
Pipeline IMPC_adult_v2-LaterOnset Screen Age (week) 11-51w
Parameter Ambulatory activity (no. of beam cuts)
Strain Condition 1 Pipeline(s) Tested Day
(Age)
IMPC_adult_v2-LaterOnset
C57BL/6NJcl Late ♀: 8  ♂: 8 2015/10/20 - 2016/07/26
(11.5 - 51.93)
Early ♀: 8  ♂: 8
Raw Data

Conditions of statistics.

sex Female

Bartlett test result
Factor df Bartlett's K2 p
condition1 1 46.17003 1.084231e-11
within 20 176.4394 4.820935e-27

Mauchly's test of sphericity
Factor Mauchly's W p

Summary statistics
Factor mean median SD SEM N
condition1 - within
Early(11w) - 1 245.5 111 258.62 91.44 8
Early(11w) - 2 141.38 47.5 232.97 82.37 8
Early(11w) - 3 187.38 216 140.85 49.8 8
Early(11w) - 4 294.75 143.5 329.73 116.58 8
Early(11w) - 5 405.62 363.5 347.78 122.96 8
Early(11w) - 6 475.12 449.5 184.89 65.37 8
Early(11w) - 7 1457.88 1300 660 233.35 8
Early(11w) - 8 2049 1923 1007.34 356.15 8
Early(11w) - 9 1902.88 1905 1009.28 356.83 8
Early(11w) - 10 1892.62 2057.5 787.54 278.44 8
Early(11w) - 11 1850 1622.5 835.55 295.41 8
Early(11w) - 12 1202 867 707.01 249.97 8
Early(11w) - 13 504.25 493 492.39 174.09 8
Early(11w) - 14 356.62 227 402.05 142.15 8
Early(11w) - 15 543.75 407 525.93 185.95 8
Early(11w) - 16 1160.38 1139 644.77 227.96 8
Early(11w) - 17 1183.25 906.5 802.29 283.65 8
Early(11w) - 18 959.62 817.5 540.87 191.23 8
Early(11w) - 19 289.62 203 318.35 112.55 8
Early(11w) - 20 525.5 542.5 388.3 137.29 8
Early(11w) - 21 105.62 77.5 113.85 40.25 8
Late(51w) - 1 237 179.5 304.09 107.51 8
Late(51w) - 2 215.88 71.5 306.3 108.29 8
Late(51w) - 3 232.88 152 242.8 85.84 8
Late(51w) - 4 297.88 120.5 457.18 161.64 8
Late(51w) - 5 343.38 285 279.65 98.87 8
Late(51w) - 6 311.38 190 255.11 90.2 8
Late(51w) - 7 811.88 626 606.63 214.47 8
Late(51w) - 8 1029 726 863.9 305.44 8
Late(51w) - 9 923.12 801 788.29 278.7 8
Late(51w) - 10 638.25 359 900.75 318.46 8
Late(51w) - 11 363.75 141 506.5 179.07 8
Late(51w) - 12 537.88 407 452.71 160.06 8
Late(51w) - 13 373.12 254 282.1 99.74 8
Late(51w) - 14 270.5 159 282.39 99.84 8
Late(51w) - 15 274.5 170 237.22 83.87 8
Late(51w) - 16 365.25 250.5 395.84 139.95 8
Late(51w) - 17 632.88 483.5 375.69 132.83 8
Late(51w) - 18 360.75 249.5 362.26 128.08 8
Late(51w) - 19 313.88 258 253.57 89.65 8
Late(51w) - 20 193.38 148.5 165.08 58.36 8
Late(51w) - 21 195.75 89 258.29 91.32 8

ANOVA result
Signif. codes ***: <0.001
**: <0.010
*: <0.050
†: <0.100
Factor df Sum Sq. Mean Sq. F value Pr(>F) Signif.
condition1 1 14785697 14785697 10.0416 0.006831 **
Residuals 14 20614247 1472446
within 20 61415945 3070797 14.5296 2.2e-16 ***
condition1:within 20 17971853 898593 4.2517 1.485e-08 ***
Residuals 280 59177354 211348

Pairwise comparisons using t tests with pooled SD
P value adjust method : Bonferroni

Factor: condition1
Pair p
Early(11w) - Late(51w) 0

Factor: within
Pair p
1 - 7 0.0065
1 - 8 0
1 - 9 0
1 - 10 4e-04
1 - 11 0.0112
2 - 7 0.0018
2 - 8 0
2 - 9 0
2 - 10 1e-04
2 - 11 0.0032
3 - 7 0.0035
3 - 8 0
3 - 9 0
3 - 10 2e-04
3 - 11 0.0061
4 - 7 0.0189
4 - 8 0
4 - 9 0
4 - 10 0.0014
4 - 11 0.0318
5 - 8 0
5 - 9 3e-04
5 - 10 0.0068
6 - 8 0
6 - 9 5e-04
6 - 10 0.0099
7 - 14 0.0261
7 - 19 0.0209
7 - 21 0.001
8 - 13 1e-04
8 - 14 0
8 - 15 0
8 - 18 0.0087
8 - 19 0
8 - 20 0
8 - 21 0
9 - 13 0.0012
9 - 14 1e-04
9 - 15 6e-04
9 - 19 1e-04
9 - 20 2e-04
9 - 21 0
10 - 13 0.0236
10 - 14 0.002
10 - 15 0.0134
10 - 19 0.0015
10 - 20 0.0051
10 - 21 1e-04
11 - 14 0.0435
11 - 19 0.0351
11 - 21 0.0018

Factor: condition1:within
Pair p
Early(11w):1 - Early(11w):10 0
Early(11w):1 - Early(11w):11 0
Early(11w):1 - Early(11w):7 0.0042
Early(11w):1 - Early(11w):8 0
Early(11w):1 - Early(11w):9 0
Early(11w):10 - Early(11w):13 2e-04
Early(11w):10 - Early(11w):14 0
Early(11w):10 - Early(11w):15 4e-04
Early(11w):10 - Early(11w):19 0
Early(11w):10 - Early(11w):2 0
Early(11w):10 - Early(11w):20 3e-04
Early(11w):10 - Early(11w):21 0
Early(11w):10 - Early(11w):3 0
Early(11w):10 - Early(11w):4 0
Early(11w):10 - Early(11w):5 0
Early(11w):10 - Early(11w):6 1e-04
Early(11w):10 - Late(51w):1 0
Early(11w):10 - Late(51w):10 0.002
Early(11w):10 - Late(51w):11 0
Early(11w):10 - Late(51w):12 3e-04
Early(11w):10 - Late(51w):13 0
Early(11w):10 - Late(51w):14 0
Early(11w):10 - Late(51w):15 0
Early(11w):10 - Late(51w):16 0
Early(11w):10 - Late(51w):17 0.0018
Early(11w):10 - Late(51w):18 0
Early(11w):10 - Late(51w):19 0
Early(11w):10 - Late(51w):2 0
Early(11w):10 - Late(51w):20 0
Early(11w):10 - Late(51w):21 0
Early(11w):10 - Late(51w):3 0
Early(11w):10 - Late(51w):4 0
Early(11w):10 - Late(51w):5 0
Early(11w):10 - Late(51w):6 0
Early(11w):10 - Late(51w):7 0.0377
Early(11w):11 - Early(11w):13 4e-04
Early(11w):11 - Early(11w):14 0
Early(11w):11 - Early(11w):15 8e-04
Early(11w):11 - Early(11w):19 0
Early(11w):11 - Early(11w):2 0
Early(11w):11 - Early(11w):20 6e-04
Early(11w):11 - Early(11w):21 0
Early(11w):11 - Early(11w):3 0
Early(11w):11 - Early(11w):4 0
Early(11w):11 - Early(11w):5 1e-04
Early(11w):11 - Early(11w):6 2e-04
Early(11w):11 - Late(51w):1 0
Early(11w):11 - Late(51w):10 0.0043
Early(11w):11 - Late(51w):11 0
Early(11w):11 - Late(51w):12 7e-04
Early(11w):11 - Late(51w):13 0
Early(11w):11 - Late(51w):14 0
Early(11w):11 - Late(51w):15 0
Early(11w):11 - Late(51w):16 0
Early(11w):11 - Late(51w):17 0.0039
Early(11w):11 - Late(51w):18 0
Early(11w):11 - Late(51w):19 0
Early(11w):11 - Late(51w):2 0
Early(11w):11 - Late(51w):20 0
Early(11w):11 - Late(51w):21 0
Early(11w):11 - Late(51w):3 0
Early(11w):11 - Late(51w):4 0
Early(11w):11 - Late(51w):5 0
Early(11w):11 - Late(51w):6 0
Early(11w):12 - Early(11w):21 0.0294
Early(11w):13 - Early(11w):8 0
Early(11w):13 - Early(11w):9 1e-04
Early(11w):14 - Early(11w):7 0.0272
Early(11w):14 - Early(11w):8 0
Early(11w):14 - Early(11w):9 0
Early(11w):15 - Early(11w):8 0
Early(11w):15 - Early(11w):9 3e-04
Early(11w):17 - Early(11w):21 0.0396
Early(11w):18 - Early(11w):8 0.0328
Early(11w):19 - Early(11w):7 0.009
Early(11w):19 - Early(11w):8 0
Early(11w):19 - Early(11w):9 0
Early(11w):2 - Early(11w):7 7e-04
Early(11w):2 - Early(11w):8 0
Early(11w):2 - Early(11w):9 0
Early(11w):20 - Early(11w):8 0
Early(11w):20 - Early(11w):9 2e-04
Early(11w):21 - Early(11w):7 3e-04
Early(11w):21 - Early(11w):8 0
Early(11w):21 - Early(11w):9 0
Early(11w):3 - Early(11w):7 0.0015
Early(11w):3 - Early(11w):8 0
Early(11w):3 - Early(11w):9 0
Early(11w):4 - Early(11w):7 0.0098
Early(11w):4 - Early(11w):8 0
Early(11w):4 - Early(11w):9 0
Early(11w):5 - Early(11w):8 0
Early(11w):5 - Early(11w):9 0
Early(11w):6 - Early(11w):8 0
Early(11w):6 - Early(11w):9 1e-04
Early(11w):7 - Late(51w):1 0.0037
Early(11w):7 - Late(51w):11 0.0304
Early(11w):7 - Late(51w):13 0.0354
Early(11w):7 - Late(51w):14 0.0065
Early(11w):7 - Late(51w):15 0.007
Early(11w):7 - Late(51w):16 0.0312
Early(11w):7 - Late(51w):18 0.029
Early(11w):7 - Late(51w):19 0.0135
Early(11w):7 - Late(51w):2 0.0025
Early(11w):7 - Late(51w):20 0.0017
Early(11w):7 - Late(51w):21 0.0018
Early(11w):7 - Late(51w):3 0.0034
Early(11w):7 - Late(51w):4 0.0104
Early(11w):7 - Late(51w):5 0.0219
Early(11w):7 - Late(51w):6 0.013
Early(11w):8 - Late(51w):1 0
Early(11w):8 - Late(51w):10 1e-04
Early(11w):8 - Late(51w):11 0
Early(11w):8 - Late(51w):12 0
Early(11w):8 - Late(51w):13 0
Early(11w):8 - Late(51w):14 0
Early(11w):8 - Late(51w):15 0
Early(11w):8 - Late(51w):16 0
Early(11w):8 - Late(51w):17 1e-04
Early(11w):8 - Late(51w):18 0
Early(11w):8 - Late(51w):19 0
Early(11w):8 - Late(51w):2 0
Early(11w):8 - Late(51w):20 0
Early(11w):8 - Late(51w):21 0
Early(11w):8 - Late(51w):3 0
Early(11w):8 - Late(51w):4 0
Early(11w):8 - Late(51w):5 0
Early(11w):8 - Late(51w):6 0
Early(11w):8 - Late(51w):7 0.0027
Early(11w):8 - Late(51w):9 0.0182
Early(11w):9 - Late(51w):1 0
Early(11w):9 - Late(51w):10 0.0017
Early(11w):9 - Late(51w):11 0
Early(11w):9 - Late(51w):12 3e-04
Early(11w):9 - Late(51w):13 0
Early(11w):9 - Late(51w):14 0
Early(11w):9 - Late(51w):15 0
Early(11w):9 - Late(51w):16 0
Early(11w):9 - Late(51w):17 0.0015
Early(11w):9 - Late(51w):18 0
Early(11w):9 - Late(51w):19 0
Early(11w):9 - Late(51w):2 0
Early(11w):9 - Late(51w):20 0
Early(11w):9 - Late(51w):21 0
Early(11w):9 - Late(51w):3 0
Early(11w):9 - Late(51w):4 0
Early(11w):9 - Late(51w):5 0
Early(11w):9 - Late(51w):6 0
Early(11w):9 - Late(51w):7 0.032

Conditions of statistics.

sex Male

Bartlett test result
Factor df Bartlett's K2 p
condition1 1 53.45129 2.650843e-13
within 20 103.9314 2.476635e-13

Mauchly's test of sphericity
Factor Mauchly's W p

Summary statistics
Factor mean median SD SEM N
condition1 - within
Early(11w) - 1 118.5 63 143.03 50.57 8
Early(11w) - 2 155.75 54 248.07 87.71 8
Early(11w) - 3 113.25 40.5 184.17 65.12 8
Early(11w) - 4 129.62 76 118.89 42.04 8
Early(11w) - 5 66.5 43.5 49.94 17.66 8
Early(11w) - 6 465.25 487 273.42 96.67 8
Early(11w) - 7 881.62 923.5 448.59 158.6 8
Early(11w) - 8 705.62 750 414.89 146.69 8
Early(11w) - 9 448 404.5 265.73 93.95 8
Early(11w) - 10 415 352.5 263.37 93.11 8
Early(11w) - 11 447.5 361.5 347.16 122.74 8
Early(11w) - 12 429.75 457 299.77 105.99 8
Early(11w) - 13 439.62 432.5 258.85 91.52 8
Early(11w) - 14 204.88 118.5 228.48 80.78 8
Early(11w) - 15 159.38 131 122.93 43.46 8
Early(11w) - 16 688 607 362.61 128.2 8
Early(11w) - 17 490.25 428.5 232.76 82.29 8
Early(11w) - 18 562.75 415.5 563.26 199.14 8
Early(11w) - 19 67.88 64.5 54.87 19.4 8
Early(11w) - 20 162 137.5 133.25 47.11 8
Early(11w) - 21 249.38 203.5 254.98 90.15 8
Late(51w) - 1 131.12 70.5 155.19 54.87 8
Late(51w) - 2 111.75 105 103.02 36.42 8
Late(51w) - 3 157.38 29 207.69 73.43 8
Late(51w) - 4 103.88 70.5 114.69 40.55 8
Late(51w) - 5 195.5 151 199.4 70.5 8
Late(51w) - 6 210 167 177.57 62.78 8
Late(51w) - 7 533.88 482.5 323.58 114.4 8
Late(51w) - 8 236.25 145.5 217.59 76.93 8
Late(51w) - 9 327.5 244 265.35 93.81 8
Late(51w) - 10 221.38 235 133.35 47.15 8
Late(51w) - 11 145.25 108.5 130.9 46.28 8
Late(51w) - 12 109.12 57 183.05 64.72 8
Late(51w) - 13 113.75 119 96.49 34.12 8
Late(51w) - 14 262.62 190 265.91 94.01 8
Late(51w) - 15 215.25 167.5 147.63 52.19 8
Late(51w) - 16 216.12 211.5 138.4 48.93 8
Late(51w) - 17 378.75 393.5 146.05 51.64 8
Late(51w) - 18 248.25 214.5 193.09 68.27 8
Late(51w) - 19 181 161 121.68 43.02 8
Late(51w) - 20 155.75 190 88.47 31.28 8
Late(51w) - 21 158.38 156.5 101.31 35.82 8

ANOVA result
Signif. codes ***: <0.001
**: <0.010
*: <0.050
†: <0.100
Factor df Sum Sq. Mean Sq. F value Pr(>F) Signif.
condition1 1 1700172 1700172 11.9073 0.003899 **
Residuals 14 1998982 142784
within 20 7616851 380843 7.4891 2.2e-16 ***
condition1:within 20 2866571 143329 2.8185 8.202e-05 ***
Residuals 280 14238852 50853

Pairwise comparisons using t tests with pooled SD
P value adjust method : Bonferroni

Factor: condition1
Pair p
Early(11w) - Late(51w) 0

Factor: within
Pair p
1 - 7 0
1 - 8 0.0352
2 - 7 0
3 - 7 0
4 - 7 0
4 - 8 0.0249
5 - 7 0
5 - 8 0.0457
6 - 7 0.0123
7 - 10 0.0051
7 - 11 0.0018
7 - 12 5e-04
7 - 13 7e-04
7 - 14 1e-04
7 - 15 0
7 - 19 0
7 - 20 0
7 - 21 0
8 - 19 0.0346

Factor: condition1:within
Pair p
Early(11w):1 - Early(11w):16 0.0018
Early(11w):1 - Early(11w):7 0
Early(11w):1 - Early(11w):8 9e-04
Early(11w):14 - Early(11w):16 0.044
Early(11w):14 - Early(11w):7 0
Early(11w):14 - Early(11w):8 0.0236
Early(11w):15 - Early(11w):16 0.0085
Early(11w):15 - Early(11w):7 0
Early(11w):15 - Early(11w):8 0.0043
Early(11w):16 - Early(11w):19 2e-04
Early(11w):16 - Early(11w):2 0.0074
Early(11w):16 - Early(11w):20 0.0094
Early(11w):16 - Early(11w):3 0.0014
Early(11w):16 - Early(11w):4 0.0027
Early(11w):16 - Early(11w):5 2e-04
Early(11w):16 - Late(51w):1 0.0029
Early(11w):16 - Late(51w):11 0.005
Early(11w):16 - Late(51w):12 0.0012
Early(11w):16 - Late(51w):13 0.0015
Early(11w):16 - Late(51w):19 0.0188
Early(11w):16 - Late(51w):2 0.0013
Early(11w):16 - Late(51w):20 0.0074
Early(11w):16 - Late(51w):21 0.0082
Early(11w):16 - Late(51w):3 0.0079
Early(11w):16 - Late(51w):4 0.001
Early(11w):16 - Late(51w):5 0.0316
Early(11w):18 - Early(11w):19 0.0291
Early(11w):18 - Early(11w):5 0.0277
Early(11w):19 - Early(11w):7 0
Early(11w):19 - Early(11w):8 1e-04
Early(11w):2 - Early(11w):7 0
Early(11w):2 - Early(11w):8 0.0038
Early(11w):20 - Early(11w):7 0
Early(11w):20 - Early(11w):8 0.0048
Early(11w):21 - Early(11w):7 1e-04
Early(11w):3 - Early(11w):7 0
Early(11w):3 - Early(11w):8 7e-04
Early(11w):4 - Early(11w):7 0
Early(11w):4 - Early(11w):8 0.0014
Early(11w):5 - Early(11w):7 0
Early(11w):5 - Early(11w):8 1e-04
Early(11w):7 - Late(51w):1 0
Early(11w):7 - Late(51w):10 0
Early(11w):7 - Late(51w):11 0
Early(11w):7 - Late(51w):12 0
Early(11w):7 - Late(51w):13 0
Early(11w):7 - Late(51w):14 2e-04
Early(11w):7 - Late(51w):15 0
Early(11w):7 - Late(51w):16 0
Early(11w):7 - Late(51w):17 0.0218
Early(11w):7 - Late(51w):18 1e-04
Early(11w):7 - Late(51w):19 0
Early(11w):7 - Late(51w):2 0
Early(11w):7 - Late(51w):20 0
Early(11w):7 - Late(51w):21 0
Early(11w):7 - Late(51w):3 0
Early(11w):7 - Late(51w):4 0
Early(11w):7 - Late(51w):5 0
Early(11w):7 - Late(51w):6 0
Early(11w):7 - Late(51w):8 1e-04
Early(11w):7 - Late(51w):9 0.0032
Early(11w):8 - Late(51w):1 0.0014
Early(11w):8 - Late(51w):10 0.0423
Early(11w):8 - Late(51w):11 0.0025
Early(11w):8 - Late(51w):12 6e-04
Early(11w):8 - Late(51w):13 7e-04
Early(11w):8 - Late(51w):15 0.0341
Early(11w):8 - Late(51w):16 0.0352
Early(11w):8 - Late(51w):19 0.0099
Early(11w):8 - Late(51w):2 7e-04
Early(11w):8 - Late(51w):20 0.0038
Early(11w):8 - Late(51w):21 0.0042
Early(11w):8 - Late(51w):3 0.004
Early(11w):8 - Late(51w):4 5e-04
Early(11w):8 - Late(51w):5 0.0168
Early(11w):8 - Late(51w):6 0.0283



F_02_U_errorbar.png M_02_U_errorbar.png

comment
Ambulatory activity (no. of beam cuts) was significantly decreased in 51-week-old mice (Late) relative to 11-week-old mice (Early) in both sexs (p<0.01).