Hematological assessment of blood determines blood cell counts (white blood cells, red blood cells, hemoglobin, and platelets) and additional hematological parameters (hematocrit, mean cell volume, mean corpuscular hemoglobin, mean cell hemoglobin concentration) can be derived using these indices. These tests will indicate abnormalities in the production of blood and its components (blood cells and hemoglobin) as well as in the associated blood-forming organs.
Description | MP Term or Comment |
---|---|
abnormal blood cell morphology/development | MP:0002429 |
Set up the hematological analyser and perform QC analyses of the control reagents in accordance with the guidelines provided by the manufacturer.
Blood collection for Clinical Chemistry and Hematology is performed as a non-fasting, terminal procedure, with some mice being used for subsequent gross pathology and other clinic-specific parameters included in terminal assessments. Whole blood (for Hematology) and plasma (for Clinical Chemistry) require different collection tubes so two independent samples are required from each mouse. Dilution of blood is highly discouraged, but is allowed when the total necessary amount is not obtained. If dilution is necessary then the assays should be done in one run.
Name | Type | Increment | Option | Unit | Data Type | Derived |
---|---|---|---|---|---|---|
White blood cell count | simpleParameter | 10^3/ul | FLOAT | |||
Red blood cell count | simpleParameter | 10^6/ul | FLOAT | |||
Hemoglobin | simpleParameter | g/dl | FLOAT | |||
Hematocrit | simpleParameter | % | FLOAT | |||
Mean cell volume | simpleParameter | fl | FLOAT | |||
Mean corpuscular hemoglobin | simpleParameter | pg | FLOAT | |||
Mean cell hemoglobin concentration | simpleParameter | g/dl | FLOAT | |||
Platelet count | simpleParameter | 10^3/ul | FLOAT | |||
Equipment ID | procedureMetadata | TEXT | ||||
Equipment manufacturer | procedureMetadata | Siemens Healthcare Diagnostics Ltd | TEXT | |||
Equipment model | procedureMetadata | ADVIA2120i | TEXT | |||
Anesthesia used for blood collection | procedureMetadata | No anesthesia | TEXT | |||
Method of blood collection | procedureMetadata | Retro-orbital puncture | TEXT | |||
Anticoagulant | procedureMetadata | K(2)-EDTA | TEXT | |||
Date and time of sacrifice | procedureMetadata | DATETIME | ||||
Blood analysis experimenter ID | procedureMetadata | TEXT | ||||
Samples kept on ice between collection and analysis | procedureMetadata | No | TEXT | |||
Mean platelet volume | simpleParameter | fl | FLOAT | |||
ID for blood collection SOP | procedureMetadata | RIKENMPP_003a_003 | TEXT | |||
Date and time of blood collection | procedureMetadata | DATETIME | ||||
Blood collection experimenter ID | procedureMetadata | TEXT | ||||
Storage temperature from blood collection until measurement | procedureMetadata | 25 | C | TEXT | ||
Red blood cell distribution width | simpleParameter | % | FLOAT | |||
Neutrophil differential count | simpleParameter | % | FLOAT | |||
Neutrophil cell count | simpleParameter | 10^3/ul | FLOAT | |||
Lymphocyte differential count | simpleParameter | % | FLOAT | |||
Lymphocyte cell count | simpleParameter | 10^3/ul | FLOAT | |||
Monocyte differential count | simpleParameter | % | FLOAT | |||
Monocyte cell count | simpleParameter | 10^3/ul | FLOAT | |||
Eosinophil differential count | simpleParameter | % | FLOAT | |||
Eosinophil cell count | simpleParameter | 10^3/ul | FLOAT | |||
Basophil cell count | simpleParameter | 10^3/ul | FLOAT | |||
Basophil differential count | simpleParameter | % | FLOAT | |||
Large Unstained Cell (LUC) count | simpleParameter | 10^3/ul | FLOAT | |||
Large Unstained Cell (LUC) differential count | simpleParameter | % | FLOAT |