To perform a complete necropsy to detect and record abnormal external findings and macroscopic alterations in internal and external organs, record body and heart weights (see IMPC Heart Weight SOP), and collect a standardized list of tissues for fixation with or without further processing (see non-mandatory IMPC Tissue Embedding & Block Banking SOP).
Banking of the fixed tissue for long-term storage is according to Centre-specific SOPs (including storage fixative). Options for long-term storage of the fixed tissue include:
Images of significant gross findings will be captured as static image files (.tiff or .jpg). Images will be not be annotated but linked by metadata to the mouse line.
Name | Type | Increment | Option | Unit | Data Type | Derived |
---|---|---|---|---|---|---|
Brain | ontologyParameter | TEXT | ||||
Eye with optic nerve | ontologyParameter | TEXT | ||||
Spinal cord | ontologyParameter | TEXT | ||||
Thymus | ontologyParameter | TEXT | ||||
Thyroid | ontologyParameter | TEXT | ||||
Heart | ontologyParameter | TEXT | ||||
Trachea | ontologyParameter | TEXT | ||||
Esophagus | ontologyParameter | TEXT | ||||
Lung | ontologyParameter | TEXT | ||||
Liver | ontologyParameter | TEXT | ||||
Gall bladder | ontologyParameter | TEXT | ||||
Stomach | ontologyParameter | TEXT | ||||
Small intestine | ontologyParameter | TEXT | ||||
Large intestine | ontologyParameter | TEXT | ||||
Pancreas | ontologyParameter | TEXT | ||||
Spleen | ontologyParameter | TEXT | ||||
Kidney | ontologyParameter | TEXT | ||||
Adrenal gland | ontologyParameter | TEXT | ||||
Mammary gland | ontologyParameter | TEXT | ||||
Lymph node | ontologyParameter | TEXT | ||||
Skin | ontologyParameter | TEXT | ||||
Skeletal muscle | ontologyParameter | TEXT | ||||
Urinary bladder | ontologyParameter | TEXT | ||||
Testes | ontologyParameter | TEXT | ||||
Epididymis | ontologyParameter | TEXT | ||||
Prostate | ontologyParameter | TEXT | ||||
Seminal vesicle | ontologyParameter | TEXT | ||||
Ovary | ontologyParameter | TEXT | ||||
Uterus | ontologyParameter | TEXT | ||||
Date of sacrifice | procedureMetadata | DATETIME | ||||
ID for sample banking protocol | procedureMetadata | RBRC_PAT_SBP_001 | TEXT | |||
Comments (in English) | simpleParameter | TEXT | ||||
Body Weight | simpleParameter | g | FLOAT | |||
Experimenter ID | procedureMetadata | TEXT | ||||
Method of sacrifice | procedureMetadata | Medetomidine/Midazolam/Butorphanol | TEXT | |||
Equipment ID | procedureMetadata | PAT-M-04 | TEXT | |||
Images | seriesMediaParameter | Minimum 1 | IMAGE | |||
Fixative | procedureMetadata | 10% buffered formalin phosphate | TEXT | |||
Equipment manufacturer | procedureMetadata | Keyence Co., Ltd | TEXT | |||
Equipment model | procedureMetadata | VHX-6000 | TEXT | |||
Salivary gland | ontologyParameter | TEXT | ||||
Sternum | ontologyParameter | TEXT | ||||
Trigeminal V nerve | ontologyParameter | TEXT | ||||
Tongue | ontologyParameter | TEXT | ||||
Bone | ontologyParameter | TEXT |