Heart Weight [EA_HWT_001]
Purpose
To evaluate cardiac size using heart weight and body weight.
Experimental Design
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Age at test: Week 17
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Sex: Both
Equipment
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Fine forceps
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Surgical scissors
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Fine surgical scissor
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Kim wipes (tissues)
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Laboratory balance
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Labelled jar with fixative
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Corkplate
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Pins
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Jar containing tap water to rinse the tools
Procedure
Methods and procedures used not including center-specific data entry methods.
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Sacrifice the mouse
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Weigh and record total body weight
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Place mouse on its back and pin the mouse onto board with extended extremities (inner side of hands and foot)
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Wipe or wet the mouse with 70% ethanol to control hair and dander
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Option A (for mice that are undergoing complete necropsy):
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Proceed with a complete necropsy and tissue collection according to Centre-specific technical SOP including removal of the heart by dissecting the aortic root immediately above the aortic valves and the superior vena cava above the atria
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Remove adjacent mediastinal fat pads from the excised heart carefully with forceps
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Empty heart blood by tapping the heart on a kim wipe (absorbent pad) or surgical compress. Repeat until the heart is totally dry
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Weigh the heart, record the weight in the Centre-specific database, and place the heart in fixative
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Option B (for mice that are not undergoing complete necropsy):
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Open the skin in the ventral midline and in the direction of the extremities and extend cut to hands and feet
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Open the muscular abdominal wall in the midline and along the lower margin of the rib cage with small forceps
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Open rib cage by removing the sternum and adjacent ribs
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Remove the heart by dissecting the aortic root immediately above the aortic valves and the superior vena cava above the atria
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Remove adjacent mediastinal fat pads from the excised heart carefully with forceps
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Empty heart blood by tapping the heart on a kim wipe (absorbent pad) or surgical compress. Repeat until the heart is totally dry
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Weigh the heart and record the weight in the Centre-specific database
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Discard the heart
© 2019 IMPC International Mouse Phenotyping Consortium.
Notes
All data are collected at a local workstation in the necropsy room (attached to a digital balance) and uploaded to the Centre-specific pathology data capture system.
Data QC
Mouse weight between 5 grams and 150 grams
IMPC Parameters (+ontology annotations)
Weight (in mg)
Data Analysis, annotation and display (+statistics)
Statistics: ANOVA using normalized heart weights (for body weight) displayed in bar graph
Parameters & Metadata
DOWNLOAD (EXCEL)
Name | Type | Increment | Option | Unit | Data Type | Derived |
Date of sacrifice | procedureMetadata | | | | DATETIME | |
Experimenter ID | procedureMetadata | | | | TEXT | |
Method of sacrifice | procedureMetadata | | Medetomidine/Midazolam/Butorphanol | | TEXT | |
Equipment ID | procedureMetadata | | PAT-B-02 / PAT-B-03 | | TEXT | |
Body weight | simpleParameter | | | g | FLOAT | |
Heart weight | simpleParameter | | | mg | FLOAT | |
Equipment manufacturer | procedureMetadata | | Sartorius AG Germany | | TEXT | |
Equipment model | procedureMetadata | | QUINTIX124-1S / BCE124I-1SJP | | TEXT | |
Heart weight normalised against body weight | simpleParameter | | | | FLOAT | Heart weight / Body weight |