Grip strength [EA_GRS_002]

Purpose

The grip strength test is used to measure the neuromuscular function as maximal muscle strength of forelimbs and combined forelimbs and hind limbs. These are assessed by the grasping applied by the mouse on a grid that is connected to a sensor. Three trials are carried out in succession measuring forelimb-strength only, followed by three successive trials measuring the combined forelimb/hindlimb grip strength. All grip strength values obtained are normalized against mouse body weight.

Description MP Term or Comment
abnormal grip strength MP:0001515

Experimental Design

Equipment

  1. Grip strength meter apparatus
  2. Balance

Procedure

  1. Set up and use of grip strength meter:
    1. Check the connection of the sensor to the grid is firmly in place to prevent the grid from turning around.
    2. Turn the sensor on and select peak mode, which will enable a measurement of the maximal strength exerted by the mouse ? the default unit of force measured is delivered in grams. Do not apply loads greater than the nominal capacity of the force sensor at the risk of permanently damaging the strength gauge.
    3. Reset the display sensor to zero.
  2. Grip strength force:
    1. Remove a mouse from its home cage, gripping the base of the tail between the thumb and the forefinger.
    2. Forelimb measurement: Lower the mouse over the grid keeping the torso horizontal and allowing only its forepaws to attach to the grid before any measurements are taken. Gently pull the mouse back by its tail ensuring the mouse grips the top portion of the grid and the torso remains horizontal and record the maximal grip strength value of the mouse that is displayed on the screen. Repeat this procedure twice more to obtain 3 forelimb grip strength measurements.
    3. Forelimb and hindlimb measurement: Lower the mouse over the grid keeping the torso parallel with the grid and allow both its forepaws and hind paws to attach to the grid before any measurements are taken. Gently pull the mouse back by its tail ensuring the torso remains parallel with the grid and record the maximal grip strength value of the mouse that is displayed on the screen. Repeat this procedure twice more to obtain 3 forelimb/hindlimb grip strength measurements.
    4. Place the mouse on the balance and record the weight of the mouse.
    5. Make a note of any further observations found during the test e.g. failure to grip the grid.
    6. Place the mouse back in its home cage.
  3. Clean the grid with ethanol (50%) and allow time to dry before testing each cage of mice.

Notes

Mice with missing digits (e.g. after toe clipping) should be omitted from this test. Behavioural parameters may be influenced by circadian rhythm therefore testing should be routinely carried out around the same time of day to obtain balanced and valid results. Avoid testing immediately after light-dark transition as behavioural outcome may be affected.

Data QC

  1. Calibrate the grip strength meter in accordance with the equipment guidelines.
  2. The force sensor is fragile and should never be overloaded.

Parameters & Metadata

DOWNLOAD (EXCEL)

Name Type Increment Option Unit Data Type Derived
Forelimb grip strength measurement seriesParameter 1, 2, 3 g FLOAT
Forelimb and hindlimb grip strength measurement seriesParameter 1, 2, 3 g FLOAT
Forelimb grip strength measurement mean simpleParameter g FLOAT Mean of Increments (Forelimb grip strength measurement)
Forelimb and hindlimb grip strength measurement mean simpleParameter g FLOAT Mean of Increments (Forelimb and hindlimb grip strength measurement)
Forelimb grip strength measurement max simpleParameter g FLOAT Max of Increments (Forelimb grip strength measurement)
Forelimb and hindlimb grip strength measurement max simpleParameter g FLOAT Max of Increments (Forelimb and hindlimb grip strength measurement)
Forelimb grip strength normalised against body weight simpleParameter FLOAT (Forelimb grip strength measurement mean/Body weight)
Forelimb and hindlimb grip strength normalised against body weight simpleParameter FLOAT (Forelimb and hindlimb grip strength measurement mean/Body weight)
Body weight simpleParameter g FLOAT
General comments about the mouse simpleParameter TEXT
Equipment ID procedureMetadata TEXT
Equipment manufacturer procedureMetadata Bioseb TEXT
Grid model procedureMetadata HMGU plate TEXT
Experimenter ID procedureMetadata TEXT
Equipment model procedureMetadata Bio-GS3 TEXT
Date equipment last calibrated procedureMetadata DATE