Welcome! NEXT-GENERATION GENE-TARGETING is for your research. [to Japanese page]


1. System Overview.

The RIKEN ENU-based gene-driven mutagenesis system (RGDMS) is open to research community as a tool of the next-generation gene-targeting within our capacity. The Mutagenesis and Genomics Team has been in charge of the RGDMS as one of the main research activities of the Team at the RIKEN BioResource Center (BRC) since April 2008, by succeeding one of the previous activities of the RIKEN Genomic Sciences Center during 2000 - 2008.The ENU-based gene-driven mutagenesis now allows us to discover about 100 - 150 ENU-induced mutations per year in various target sequences of the mouse genome. Key information to use the RGDMS is also written in the application form below.

Please note: Due to the mating scheme to produce the G1males, no X-linked genes are subjected to the ENU-based gene-driven mutagenesis. (No ENU-treated X chromosomes are inherited to G1 males.)

BRC Experimental Animal Division will distribute the discovered mutant strain to the user from the frozen sperm. It costs JPN¥98,600 (for non-profit organization)-JPN¥197,200 (for profit organizationa) plus tax per strain as of March 19, 2010. The cost may be subjected to change. Please see the BRC Experimental Animal Division Page for the distribution costs.

2. Flow of the USE

(Further information are available in the Application Form.)

3. Target Gene List.

4. Available Mutant Mouse Strains established by ENU-based gene-driven mutagenesis.

5. Application Form/PCR Design Manual, etc.

Further inquiry to;


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