研究成果

Home > 研究成果

論文掲載

Nat Commun. 2013 Apr 23;4:1754.  Max is a repressor of germ cell-related gene expression in mouse embryonic stem cells.
Maeda I, Okamura D, Tokitake Y, Ikeda M, Kawaguchi H, Mise N, Abe K, Noce T, Okuda A, Matsui Y. 
Cell Stem Cell. 2013 Mar 7;12(3):368-82. PRDM14 Ensures Naive Pluripotency through Dual Regulation of Signaling and Epigenetic Pathways in Mouse Embryonic Stem Cells.
Masashi Yamaji, Jun Ueda, Katsuhiko Hayashi, Hiroshi Ohta, Yukihiro Yabuta, Kazuki Kurimoto, Ryuichiro Nakato, Yasuhiro Yamada, Katsuhiko Shirahige and Mitinori Saitou.  
Genome Res. 2013 Feb;23(2):292-9.  Targeted gene silencing in mouse germ cells by insertion of a homologous DNA into a piRNA generating locus.
Yamamoto, Y., Watanabe, T., Hoki, Y., Shirane, K., Li, Y., Ichiiyanagi, K., Kuramochi-Miyagawa, S., Toyoda, A., Fujiyama, A., Oginuma, M., Suzuki, H., Sado, T., Nakano, T. and Sasaki, H.  
Genes Dev.2012 Nov 15;26(22):2477-82.   Cell cycle gene-specific control of transcription has a critical role in proliferation of primordial germ cells.
Daiji Okamura, Ikuma Maeda, Hirofumi Taniguchi, Yuko Tokitake, Makiko Ikeda, Keiko Ozato, Nathan Mise, Kuniya Abe, Toshiaki Noce, Juan Carlos Izpisua Belmonte, and Yasuhisa Matsui. 
Cell Reports. 2012 Nov 29;2(5):1363-74.  Molecular identification of tw5Vps52 promotes pluripotential cell differentiation through cell-cell interactions.
Michihiko Sugimoto, Masayo Kondo, Michiko Hirose, Misao Suzuki, Kazuyuki Mekada, Takaya Abe, Hiroshi Kiyonari, Atsuo Ogura, Nobuo Takagi, Karen Artzt, and Kuniya Abe.
Science. 2012 Nov 16;338(6109):971-5 Offspring from Oocytes Derived from in Vitro Primordial Germ Cell–Like Cells in Mice. 
Katsuhiko Hayashi, Sugako Ogushi, Kazuki Kurimoto, So Shimamoto, Hiroshi Ohta, Mitinori Saitou.
Cell Stem Cell. 2012 Oct 5;11(4):567-78. Reconstitution of Mouse Spermatogonial Stem Cell Niches in Culture. 
Mito Kanatsu-Shinohara, Kimiko Inoue, Seiji Takashima, Masanori Takehashi, Narumi Ogonuki, Hiroko Morimoto, Takashi Nagasawa, Atsuo Ogura, and Takashi Shinohara.
Nature. 2012 Jun 3;486(7403):415-9. PGC7 binds histone H3K9me2 to protect against conversion of 5mC to 5hmC in early embryos.
Toshinobu Nakamura, Yu-Jung Liu, Hiroyuki Nakashima, Hiroki Umehara, Kimiko Inoue, Shogo Matoba, Makoto Tachibana, Atsuo Ogura, Yoichi Shinkai & Toru Nakano.
Proc Natl Acad Sci U S A. 2012 Mar 6;109(10):3850-5. Protein disulfide isomerase homolog PDILT is required for quality control of sperm membrane protein ADAM3 and male infertility.
Keizo Tokuhiro, Masahito Ikawa, Adam M. Benham, and
Masaru Okabe.
Proc Natl Acad Sci U S A. 2011 Dec 13;108(50):20008-11. Acrosome-reacted mouse spermatozoa recovered from the perivitelline space can fertilize other eggs.
Naokazu Inoue, Yuhkoh Satouh, Masahito Ikawa,
Masaru Okabe, and Ryuzo Yanagimachi.
Proc Natl Acad Sci U S A. 2011 Dec 20;108(51):20621-6. RNAi-mediated knockdown of Xist can rescue the impaired postimplantation development of cloned mouse embryos.
Shogo Matoba, Kimiko Inoue,
Takashi Kohda, Michihiko Sugimoto, Eiji Mizutani, Narumi Ogonuki, Toshinobu Nakamura, Kuniya Abe, Toru Nakano, Fumitoshi Ishino, and Atsuo Ogura. 
Cell Stem Cell. 2011 Nov 4;9(5):463-75. Rac Mediates Mouse Spermatogonial Stem Cell Homing to Germline Niches by Regulating Transmigration through the Blood-Testis Barrier.
Seiji Takashima, Mito Kanatsu-Shinohara, Takashi Tanaka, Masanori Takehashi, Hiroko Morimoto, and
Takashi Shinohara.
Genome Res. 2011 Dec;21(12):2058-66. Locus- and domain-dependent control of DNA methylation at mouse B1 retrotransposons during male germ cell development.
Ichiyanagi K, Li Y, Watanabe T, Ichiyanagi T, Fukuda K, Kitayama J, Yamamoto Y, Kuramochi-Miyagawa S, Nakano T, Yabuta Y, Seki Y, Saitou M, Sasaki H.
Cell. 2011 Aug 19;146(4):519-32. Reconstitution of the Mouse Germ Cell Specification Pathway in Culture by Pluripotent Stem Cells.
Katsuhiko Hayashi, Hiroshi Ohta, Kazuki Kurimoto, Shinya Aramaki, and Mitinori Saitou.
Proc Natl Acad Sci U S A. 2011 Jun 28;108(26):10579-84. Tudor domain containing 7 (Tdrd7) is essential for dynamic ribonucleoprotein (RNP) remodeling of chromatoid bodies during spermatogenesis.
Takashi Tanaka, Mihoko Hosokawa, Vasily V. Vagin, Michael Reuter, Eri Hayashi, Ayako L. Mochizuki, Kouichi Kitamura, Hidenori Yamanaka, Gen Kondoh, Katsuya Okawa, Satomi Kuramochi-Miyagawa,
Toru Nakano, Ravi Sachidanandam, Gregory J. Hannon, Ramesh S. Pillai, Norio Nakatsuji, and Shinichiro Chuma.
Science. 2011 May 13;332(6031):848-52.
Role for piRNAs and non-coding RNA in de novo DNA methylation of the  
imprinted mouse Rasgrf1 locus.

Watanabe, T., Tomizawa, S., Mitsuya, K., Totoki, Y., Yamamoto, Y., Kuramochi-Miyagawa, S., Iida, N., Hoki, Y., Murphy, P.J., Toyoda, A.,  Gotoh, K., Hiura,H., Arima, T., Fujiyama, A., Sado, T., Shibata, T.,  Nakano, T., Lin, H., Ichiyanagi, K., Soloway, P.D. & Sasaki, H.
Dev Cell.
2011 Mar 15; 20(3):364-75.
 
MitoPLD Is a Mitochondrial Protein Essential for Nuage Formation and piRNA Biogenesis in the Mouse Germline.
Toshiaki Watanabe,
Shinichiro Chuma, Yasuhiro Yamamoto, Satomi Kuramochi-Miyagawa, Yasushi Totoki, Atsushi Toyoda, Yuko Hoki, Asao Fujiyama, Tatsuhiro Shibata, Takashi Sado, Toshiaki Noce, Toru Nakano, Norio Nakatsuji, Haifan Lin and Hiroyuki Sasaki.
Science
2010 Oct 22; 330(6003):496-9.
Impeding Xist Expression from the Active X Chromosome Improves Mouse Somatic Cell Nuclear Transfer.
Kimiko Inoue, Takashi Kohda, Michihiko Sugimoto, Takashi Sado, Narumi Ogonuki1, Shogo Matoba1, Hirosuke Shiura1, Rieko Ikeda1, Keiji Mochida1, Takashi Fujii,
Ken, Sawai, Arie P. Otte, X Cindy Tian, Xiangzhong Yang, Fumitoshi Ishino, Kuniya Abe, Atsuo Ogura.
Genes Dev.
2010 May;24(9):887-92.
 
MVH in piRNA processing and gene silencing of retrotransposons.
Satomi Kuramochi-Miyagawa, Toshiaki Watanabe, Kengo Gotoh, Kana Takamatsu,
Shinichiro Chuma, Kanako Kojima-Kita, Yusuke Shiromoto, Noriko Asada,Atsushi Toyoda, Asao Fujiyama, Yasushi Totoki, Tatsuhiro Shibata, Tohru Kimura, Norio Nakatsuji, Toshiaki Noce, Hiroyuki Sasaki, Toru Nakano.
Proc Natl Acad Sci U S A.
2010 Apr 6; 107(14):6210-5.
Transmission distortion by loss of p21 or p27 cyclin-dependent kinase inhibitors following competitive spermatogonial transplantation.
Mito Kanatsu-Shinohara, Seiji Takashima  & 
Takashi Shinohara. 
Nature.
2010 Apr 8; 464(7290):927-31.
 
Proviral silencing in embryonic stem cells requires the histone methyltransferase ESET.
Toshiyuki Matsui, Danny Leung, Hiroki Miyashita, Irina A. Maksakova, Hitoshi Miyachi, Hiroshi Kimura, Makoto Tachibana, Matthew C. Lorincz &
Yoichi Shinkai.
Dev Cell.
2009 Dec; 17(6):775-87.
 
The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline.
Masanobu Shoji, Takashi Tanaka, Mihoko Hosokawa, Michael Reuter, Alexander Stark Yuzuru Kato, Gen Kondoh, Katsuya Okawa, Takeshi Chujo, Tsutomu Suzuki,
Kenichiro Hata, Sandra L. Martin, Toshiaki Noce, Satomi Kuramochi-Miyagawa, Toru Nakano, Hiroyuki Sasaki, Ramesh S. Pillai, Norio Nakatsuji, and Shinichiro Chuma. 
Nature.
2009 Oct 29; 461(7268):1296-9.
 

A regulatory circuit for piwi by the large Maf gene traffic jam in Drosophila.
Kuniaki Saito,  Sachi Inagaki,  Toutai Mituyama,  Yoshinori Kawamura,  Yukiteru Ono,  Eri Sakota,  Hazuki Kotani,  Kiyoshi Asai,  Haruhiko Siomi  &  Mikiko C. Siomi.

Cell Stem Cell.
2009 Jul 2; 5(1):76-86.
Genetic Reconstruction of Mouse Spermatogonial Stem Cell Self-Renewal In Vitro by Ras-Cyclin D2 Activation.
Jiyoung Lee, Mito Kanatsu-Shinohara, Hiroko Morimoto, Yasuhiro Kazuki, Seiji Takashima, Mitsuo Oshimura, Shinya Toyokuni, and
Takashi Shinohara. 
Cell.
2009 May 1; 137(3):571-84.
 
A signaling principle for the specification of the germ cell lineage in mice.
Ohinata, Y., Ohta, H., Shigeta, M., Yamanaka, K.,
Wakayama, T., Saitou, M. 
Cell Stem Cell.
2008 Nov 6; 3(5):533-42.
Homing of mouse spermatogonial stem cells to germline niche depends of beta1-integrin.
Mito Kanatsu-Shinohara, Masanori Takehashi, Seiji Takashima, Jiyoung Lee, Hiroko Morimoto, Shinichiro Chuma, Aurelia Raducanu, Norio Nakatsuji, Reinhard Fassler and Takashi Shinohara.
 
Nat Genet.
2008 Aug; 40(8):1016-22.
 
Critical function of Prdm14 for the establishment of the germ cell lineage in mice.
Yamaji, M., Seki, Y., Kurimoto, K., Yabuta, Y., Yuasa, M., Shigeta, M, Yamanaka, K., Ohinata, Y., and Saitou, M.
Genes Dev. 2008 Jun 15;22(12):1617-35.  Complex genome-wide transcription dynamics orchestrated by Blimp1 for the specification of the germ cell lineage in mice.
Kazuki Kurimoto, Yukihiro Yabuta, Yasuhide Ohinata, Mayo Shigeta, Kaori Yamanaka, and Mitinori Saitou.
 
Genes Dev. 2008 Jun 15;22(12):1607-16 Maternal and zygotic Dnmt1 are necessary and sufficient for the maintenance of DNA methylation imprints during preimplantation development.
Ryutaro Hirasawa, Hatsune Chiba, Masahiro Kaneda, Shoji Tajima, En Li, Rudolf Jaenisch, and
Hiroyuki Sasaki. 
Nature. 2008 May 22;453(7194):539-43. Endogenous siRNAs from naturally formed dsRNAs regulate transcripts in mouse oocytes.
Toshiaki Watanabe, Yasushi Totoki, Atsushi Toyoda, Masahiro Kaneda, Satomi Kuramochi-Miyagawa, Yayoi Obata, Hatsune Chiba,
Yuji Kohara, Tomohiro Kono, Toru Nakano, M. Azim Surani, Yoshiyuki Sasaki, and Hiroyuki Sasaki. 
Genes Dev.
2008 Apr 1; 22(7):908-17.
 
DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes.
Satomi Kuramochi-Miyagawa, Toshiaki Watanabe, Kengo Gotoh, Yasushi Totoki, Atsushi Toyoda, Masahito Ikawa, Noriko Asada, Kanako Kojima, Yuka Yamaguchi, Takashi W. Ijiri,
Kenichiro Hata, En Li, Yoichi Matsuda, Tohru Kimura, Masaru Okabe, Yoshiyuki Sakaki, Hiroyuki Sasaki, and Toru Nakano.